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A new cryptic species of the genus isolated from the bark of a dead pine tree was characterized using morphological features, morphometrics, and DNA barcodes. n. sp. is characterized by its stoma 20-24 μm in depth; tips of prorhabdions not bent inwards; metarhabdions with two subventral, and two subdorsal teeth; corpus longer than postcorpus; hemizonid 15.0-26.5 μm posterior to excretory pore; vulva-anus distance 21.5-31.5 μm, ca equal to or slightly less than vulval body diameter; rectum distinctly longer than anal body diameter; female tail cupola-shaped, conoid posteriorly, with an extended spike; male with slender spicules, nearly straight to minimally curved towards a nearly acute to a bluntly rounded tip; and bursa with 10 pairs of bursal rays, with a 2 + 3 + 2 + 3 typical pattern. It differs from the morphologically similar by the non-bent tips of prorhabdions, the corpus being longer than postcorpus, the bursal rays' pattern, and a more cupola-shaped tail in female and DNA barcodes. The DNA phylogenies using the 18S rRNA, 28S rRNA and gene markers showed well-supported sister relations of n. sp. with and .
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http://dx.doi.org/10.2478/jofnem-2025-0027 | DOI Listing |
Anal Chim Acta
November 2025
Department of Clinical Laboratory Medicine, Shanghai Tenth People's Hospital, School of Medicine, Tongji University, Shanghai, 200072, PR China.
Background: A DNA barcode is a short DNA fragment used to classify and identify specific organisms, taking advantage of the specificity and diversity inherent in biological molecules. Since Herbert introduced the concept in 2003, DNA barcoding has been increasingly used in precision medicine and related fields, including species identification and environmental monitoring, over the past few decades. Although numerous molecular diagnostic techniques have emerged, many face notable obstacles such as sensitivity to handling conditions, high expenses, and limitations in accuracy.
View Article and Find Full Text PDFZookeys
August 2025
Ecology and Genetics Research Unit, P.O. Box 3000, 90014 University of Oulu, Oulu, Finland University of Oulu Oulu Finland.
A male of the little-known species Sruoga & Kaila, 2019 is described for the first time based on material collected in northern Thailand. The species is diagnosed based on characters found in the habitus and genitalia, which are illustrated in detail. Conspecificity of male and female specimens is confirmed by DNA barcodes.
View Article and Find Full Text PDFBlood Transfus
August 2025
EFS BloodCenter of Brittany, HLA-HPA Laboratory, Rennes, France.
Background: Non-invasive fetal HPA typing is a valuable tool to identify the pregnancies at risk of fetal and neonatal alloimmune thrombocytopenia (FNAIT). Different approaches have been developed, mainly based on real-time PCR and droplet digital-PCR. Those methods have a limited ability to multiplex and require replicates due to the contamination risk.
View Article and Find Full Text PDFSci Rep
September 2025
Department of Biosciences and Territory, University of Molise, Pesche, Italy.
Accurate species identification, the first crucial step for effective root studies, is a time-demanding, experience-based and error-prone process. Molecular methods are therefore needed to ensure this process, especially in urban settings where root sampling is challenging. Here, we developed a novel molecular method for root identification in complex environments.
View Article and Find Full Text PDFJ Nematol
February 2025
Research Institute of Invertebrate Vector, Kyungpook National University, Sangju 37224, Republic of Korea.
A new cryptic species of the genus isolated from the bark of a dead pine tree was characterized using morphological features, morphometrics, and DNA barcodes. n. sp.
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