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Article Abstract

A new cryptic species of the genus isolated from the bark of a dead pine tree was characterized using morphological features, morphometrics, and DNA barcodes. n. sp. is characterized by its stoma 20-24 μm in depth; tips of prorhabdions not bent inwards; metarhabdions with two subventral, and two subdorsal teeth; corpus longer than postcorpus; hemizonid 15.0-26.5 μm posterior to excretory pore; vulva-anus distance 21.5-31.5 μm, ca equal to or slightly less than vulval body diameter; rectum distinctly longer than anal body diameter; female tail cupola-shaped, conoid posteriorly, with an extended spike; male with slender spicules, nearly straight to minimally curved towards a nearly acute to a bluntly rounded tip; and bursa with 10 pairs of bursal rays, with a 2 + 3 + 2 + 3 typical pattern. It differs from the morphologically similar by the non-bent tips of prorhabdions, the corpus being longer than postcorpus, the bursal rays' pattern, and a more cupola-shaped tail in female and DNA barcodes. The DNA phylogenies using the 18S rRNA, 28S rRNA and gene markers showed well-supported sister relations of n. sp. with and .

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399297PMC
http://dx.doi.org/10.2478/jofnem-2025-0027DOI Listing

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