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Article Abstract
High-performance anion-exchange chromatography with acid eluents allows for effective separation of myo-inositol tetrakisphosphates, myo-inositol pentakisphosphates, diphospho-myo-inositol phosphates (inositol pyrophosphates), inositol hexakisphosphates, inositol pyrophosphate analogs, and carbohydrate-based analogs of inositol phosphates. All are detectable by postcolumn addition of ferric ion. In the following, we provide examples drawn from characterization of ITPK, IPK1, and IP 3K enzymes.
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