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Article Abstract
Background: This study aims to develop a thermo-photo dual-stimuli-responsive hydrogel matrix and ROS-responsive engineered nanocarriers-based composite GelPol nanoformulation for corneal wound management. GelPol is composed of modified gelatin and poloxamer 407SH holding nanoparticles (NPs) loaded with dexamethasone, rapamycin, and ciprofloxacin.
Research Design And Methods: Dual-stimuli-responsive hydrogel and ROS-responsive NPs were synthesized and characterized. Sol-gel transition of GelPol was studied at various temperatures and light irradiation. In vitro release studies and kinetics from the GelPol nanoformulation were conducted at 25°C and 37°C over 3 weeks. Structural integrity and stability studies were performed under various conditions (temperature and pH) for 4 weeks and confirmed through FTIR and DSC analyses. Cell viability and biocompatibility studies using HCEC were conducted to assess safety.
Results: The NPs characterization revealed hydrodynamic diameters ranging from 105 to 114 nm with polydispersity between 0.108 and 0.153. Encapsulation efficiencies for DEX, RAPA, and CIP were 87.27%, 88.11%, and 76.94%, respectively. Stability studies confirmed the GelPol stability through FTIR, DSC, and HPLC analyses. Cell viability and biocompatibility studies demonstrated the safety of GelPol formulations.
Conclusions: The tailored GelPol novel approach could offer a noninvasive alternative for corneal wound treatment, potentially improving patient safety and adherence compared to traditional procedures.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12413028 | PMC |
| http://dx.doi.org/10.1080/17435889.2025.2550236 | DOI Listing |
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