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Surplus bread accounts for a significant proportion of food waste in many countries. The focus of this study was twofold: firstly, to investigate the use of carasau bread residue as a sourdough substrate, and secondly, to reuse this sourdough into a new carasau baking process. Selected lactic acid bacteria () and yeast strains ( and ) were used to inoculate three substrates: bread residue (S1), bread residue supplemented with durum wheat middlings (S2), and semolina (S3). Sourdoughs were refreshed for five days by backslopping, and microbiological and physicochemical analyses were performed. Results indicated that incorporating wheat middlings into bread residue enhanced microbial performance, as evidence by a decrease in pH from 6.0 to around 4.5 compared to using bread residue alone as a substrate. Carasau bread produced with the sourdough derived from bread residue and wheat middlings exhibited comparable physicochemical properties to commercial baker's yeast carasau bread, but had better sensory properties, scoring a mean acceptability of 7.0 versus 6.0 for baker's yeast bread. These results show that bread residue supplemented with wheat middlings can serve as a sourdough substrate, allowing its reuse in the baking process to produce high-quality carasau bread and promote the circular economy.
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http://dx.doi.org/10.3390/microorganisms13081745 | DOI Listing |
Microorganisms
July 2025
Porto Conte Ricerche, Loc. Tramariglio, 07041 Alghero, Italy.
Surplus bread accounts for a significant proportion of food waste in many countries. The focus of this study was twofold: firstly, to investigate the use of carasau bread residue as a sourdough substrate, and secondly, to reuse this sourdough into a new carasau baking process. Selected lactic acid bacteria () and yeast strains ( and ) were used to inoculate three substrates: bread residue (S1), bread residue supplemented with durum wheat middlings (S2), and semolina (S3).
View Article and Find Full Text PDFPlant Physiol Biochem
June 2025
Department of Botany, Panjab University, Chandigarh, 160014, India. Electronic address:
Mitochondrial calcium uniporters (MCUs) play a crucial role in calcium transport, signaling, and homeostasis, which is vital for plant growth, development, and stress responses. In this study, we identified 17 TaMCU genes classified into seven homoeologous groups with conserved gene architecture in the bread wheat genome. The structural analysis revealed the presence of MCU domain, DVME motif, transmembrane helices, and various key amino acid residues, which are conserved across all TaMCU proteins.
View Article and Find Full Text PDFBMC Plant Biol
May 2025
State Key Laboratory of Wheat and Maize Crop Science, Collaborative Innovation Center of Henan Grain Crops, College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, China.
Background: Lignin, the second most abundant terrestrial biopolymer, represents a significant renewable natural biomaterial. Cinnamoyl-CoA reductase (CCR) catalyzes the conversion of various hydroxycinnamoyl-CoA esters into their corresponding aldehydes, utilizing NADPH as a cofactor. CCR functions as a regulatory point that controls the overall carbon flux towards lignin and constitutes the initial committed step in the lignin biosynthesis pathway.
View Article and Find Full Text PDFJ Inorg Biochem
September 2025
Department of Chemistry, University of Zurich, Zurich, Switzerland. Electronic address:
Metallothioneins (MTs) are small cysteine-rich proteins that preferentially bind d metal ions such as Zn, Cu, and Cd, playing essential roles in metal ion homeostasis and detoxification. The E-1 metallothionein from Triticum aestivum (common bread wheat) was the first plant metallothionein for which a 3D structure was successfully determined, although this structure represents only the fully metalated state of the protein. In this study, we aim to elucidate the metalation pathway of the β-domain of wheat E-1.
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