Alleviation of Ovalbumin-Allergic Reactions in Mice by Polysaccharides via Modulation of Intestinal Microbiota.

Food allergy represents a prevalent immunological disorder, with current clinical management primarily emphasizing allergen avoidance and emergency pharmacological intervention. polysaccharides, the principal bioactive constituents of the traditional Chinese medicinal plant , have demonstrated anti-inflammatory and antioxidant properties; however, their specific effects on food allergies remain inadequately characterized. A total of thirty-six female BALB/c mice were randomly allocated into three groups ( = 12 per group): the control group (CON group, receiving saline treatment), the allergic model group (OVA group, subjected to ovalbumin sensitization), and the intervention group (OVA+PS group, undergoing OVA sensitization followed by polysaccharides administration via gavage). The anti-allergic efficacy of polysaccharides was comprehensively evaluated through clinical allergy symptom scoring, histological and pathological tissue analysis, real-time fluorescence quantitative PCR (qRT-PCR) for the assessment of key gene expression, and 16S rDNA sequencing. The findings indicated the following: (1) The allergy scores in the OVA+PS group were significantly lower than those in the OVA group ( < 0.01). Following OVA stimulation, the rectal temperature of mice in the OVA group decreased sharply, whereas the temperature decline in the OVA+PS group was more gradual compared to the model group. (2) The liver, kidney, spleen, and intestinal tissues of mice in the OVA+PS group exhibited normal morphology, consistent with the CON group, which suggests that polysaccharides effectively mitigates the local inflammatory response induced by food allergy. (3) The expression of in the spleen of mice in the OVA+PS group was significantly higher than in the OVA group ( < 0.05), while the expression of the gene was significantly elevated in the OVA group compared to both the CON and OVA+PS groups ( < 0.05). In the OVA group, the expression level of was significantly elevated compared to both the CON group and the OVA+PS group ( < 0.05). Similarly, the expression of in the OVA group was markedly higher than in the other groups ( < 0.05). (4) polysaccharides were found to modulate the intestinal microbiota composition in allergic mice, notably increasing the expression abundance of , , and , while decreasing the expression abundance of and . (5) There was a correlation between alterations in the intestinal microbiota of mice and the expression of key genes. Specifically, the relative abundance of was negatively correlated with the expression of and genes ( < 0.05), and the relative abundance of the was negatively correlated with the expression of the 1 gene ( < 0.05). In conclusion, polysaccharides demonstrate potential in alleviating allergic symptoms, providing a scientific foundation for the development of novel natural anti-allergic functional foods.