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Article Abstract
Mammalian genomes undergo pervasive transcription in both genic and intergenic regions. Trimethylation of histone H3 lysine 4 (H3K4me3) is a deeply conserved and functionally important histone modification enriched at transcriptionally active promoters, where it facilitates RNA polymerase activity. H3K4me3 is also commonly found in intergenic regions, where its role is poorly understood. We interrogated the role of H3K4me3 at intergenic regulatory elements by using epigenetic editing to efficiently deposit H3K4me3 at intergenic loci. We found that H3K4me3 amplifies RNA polymerase activity and is actively remodeled at intergenic regions, shedding light on these important but poorly understood regions of the genome.
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Article Synopsis
- Nuclear insertions of mitochondrial DNA segments (NUMTs) are dynamic genetic elements that arise from the ancient relationship between mitochondria and host cells, primarily found in areas of the genome linked to regulatory functions.
- Recent research shows that stress-induced mitochondrial damage can trigger the release of mtDNA, leading to NUMT integration, which may be more common in aging tissues and linked to cognitive decline and cancer development.
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