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Article Abstract
Nanobodies specifically recognizing atrazine were successfully obtained through immunization of alpacas and phage display libraries, and an indirect competitive immunoassay (ic-ELISA) based on nanobodies was constructed with an IC of 0.062 μg/mL and a minimum detection limit of 0.01 μg/mL. An Au@PtNPs with excellent colorimetric performance, high catalytic activity, and high stability was synthesized. The combination of Au@PtNPs and nanobodies led to the development of a direct competitive immunoassay (dc-ELISA) with higher specificity, convenience, lower cost and an IC value of 0.032 μg/mL which was lower than the ic-ELISA. Based on the Au@PtNPs and nanobodies, a novel LFIA model for qualitative and semi-quantitative detection of atrazine was constructed with a visual detection limit of 5 ng/mL. The present study is of great significance for the construction of atrazine immunoassay combining nanobodies and nanoenzymes, which provides a sensitive, specific, simple and reliable method for the detection of atrazine in the environment.
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Development of an atrazine immunoassay based on highly catalytically active Au@PtNPs and specific nanobody.
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Nanobodies specifically recognizing atrazine were successfully obtained through immunization of alpacas and phage display libraries, and an indirect competitive immunoassay (ic-ELISA) based on nanobodies was constructed with an IC of 0.062 μg/mL and a minimum detection limit of 0.01 μg/mL.
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Article Synopsis
- Scientists are using special tests called immunoassays to find tiny amounts of chemicals, like pesticides, in food and the environment.
- They created new types of antibodies specifically for a pesticide called atrazine, which helps make more accurate tests and reduces mistakes.
- The new test showed good results in spotting atrazine in fruits, veggies, and tea, proving it can help keep our food safer from harmful chemicals.
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Heliyon
January 2024
Department of Biochemistry, University of Port Harcourt, Choba, Nigeria.
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