Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Research on chemokines in cattle is hampered by the relative lack of validated reagents. Bovine IP-10 is an important inflammatory chemokine and a promising diagnostic biomarker for an economically important disease (bovine tuberculosis) caused by infection of cattle with Mycobacterium bovis. Currently no monoclonal antibodies are available for bovine IP-10. The goal of this study was to generate novel mAbs for detection of bovine IP-10 using hybridoma technology. Five mAbs were developed and cross clone inhibition analyses showed a high degree of self-inhibition among the mAbs. One mAb (7C2) was used in conjugation with a commercial polyclonal antibody to develop a sandwich ELISA. Upon testing with recombinant bovine IP-10, this ELISA showed an enhanced linear range compared to the currently available polyclonal antibody-based ELISA. The ELISA using 7C2 was shown to detect native antigen-specific bovine IP-10 in samples from M. bovis infected animals. The 7C2 mAb could also detect intracytoplasmic IP-10. These novel mAbs will be useful in elucidating roles for IP-10 in bovine immune studies in health, disease and vaccination contexts.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12351912 | PMC |
| http://dx.doi.org/10.1186/s13567-025-01602-z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Development and characterization of monoclonal antibodies specific for bovine IP-10.
Vet Res
August 2025
Division of Immunology, The Roslin Institute, University of Edinburgh, Edinburgh, EH25 9RG, UK.
Research on chemokines in cattle is hampered by the relative lack of validated reagents. Bovine IP-10 is an important inflammatory chemokine and a promising diagnostic biomarker for an economically important disease (bovine tuberculosis) caused by infection of cattle with Mycobacterium bovis. Currently no monoclonal antibodies are available for bovine IP-10.
View Article and Find Full Text PDFTranscriptomic insights into infection-associated pulmonary granulomas reveal multicellular immune networks and tuberculosis biomarkers in cattle.
Vet Q
December 2025
National Institute of Animal Biotechnology, Hyderabad, Telangana, India.
, a member of the complex (MTBC), has emerged as a significant contributor to tuberculosis (TB) in cattle, wildlife, and humans. However, understanding about its pathogenesis and severity is limited, compounded by the lack of reliable TB biomarkers in cattle. This study delves into the comparative pathology and transcriptomic landscape of pulmonary granulomas in cattle naturally infected with , using high-throughput RNA sequencing.
View Article and Find Full Text PDFCytokines as potential biomarkers for the diagnosis of infection in Mediterranean buffaloes ().
Front Vet Sci
December 2024
National Reference Centre for Hygiene and Technologies of Mediterranean Buffalo Farming and Productions, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Salerno, Italy.
() is the primary agent of bovine tuberculosis (TB) in Mediterranean buffalo, which has a negative economic impact on buffalo herds. Improving TB diagnostic performance in this species represents a key step to eradicate efficiently this disease. We have recently shown the utility of the IFN-γ assay in the diagnosis of infection in Mediterranean buffaloes (), but other cytokines might be useful immunological biomarkers of this infection.
View Article and Find Full Text PDFOral paratuberculosis vaccine efficacy and mucosal immunity in cattle.
Vaccine
December 2024
Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada. Electronic address:
Mycobacterium avium subsp. paratuberculosis (MAP) primarily invades ruminants' small intestine via the Peyer's patches in the ileum and jejunum. Despite ongoing efforts to develop effective MAP vaccines, the effects of live-attenuated vaccines on mucosal immunity remain poorly understood.
View Article and Find Full Text PDFInfluence of commercial inactivated or modified-live virus vaccination at time of AI on corpus luteum development and function in beef cattle.
Anim Reprod Sci
November 2024
Texas A&M AgriLife Research, Overton, TX 75684, USA. Electronic address:
Our objective was to evaluate the effect of vaccination with an inactivated virus vaccine (IVV) or modified-live virus (MLV) vaccine on the corpus luteum (CL). On d0, synchronized beef cows were treated with MLV (n = 70; BoviShield Gold FP5VL5), IVV (n = 16; ViraShield 6VL5HB), or were unvaccinated controls (n = 5). Plasma was collected from treated animals on d0 and every other day through d22.
View Article and Find Full Text PDF