Recognition of the impact of mycelium extract on ethanol-damaged gastric epithelial cells using atomic force microscopy.

Atomic force microscopy (AFM) is used to measure live cells. Changes in the biomechanical properties of a single cell under mycelium extract () treatment play a critical role in drug efficacy evaluation and drug development, but they remain underexplored. In this study, ethanol-damaged human gastric mucosal cells (GES-1) treated with were examined through AFM to investigate the physical changes. GES-1 cells were exposed to 5% ethanol to create a cell damage model. -induced physical changes in the cell damage model were evaluated using AFM, including cell surface morphology, adhesion force, Young's modulus, and cell surface roughness. For comparison, the GES-1 cells were treated with various concentrations of as a normal group. The scratch assay and colony-forming assay were conducted to determine the growth-promoting effects of . The results show that 200 μg mL of is the optimal dosage, exhibiting the best repair capacity. This study presents a single-cell approach for evaluating the drug efficacy of , and the methodology used in this study is expected to investigate the effects of other drugs and physical and chemical factors.