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The replacement of animal fat with unsaturated lipid sources in processed meats enhances nutritional value but introduces challenges regarding oxidative stability and sensory acceptability. In this study, the effects of replacing pork back fat with pre-emulsified walnut, linseed, or algal oils on the proximate composition, fatty acid profile, nutritional indices, lipid oxidation, and sensory properties of chicken frankfurters were investigated. Four formulations were prepared: a control group (25% pork fat) and three groups that were completely reformulated using oil emulsions (ratio inulin/water/oil 1:2:1). The fat substitute significantly reduced total fat, SFA, cholesterol (up to 30%), and calorie density, while Ʃn-3 fatty acids were enriched ( < 0.05). The linseed oil samples had the highest levels of α-linolenic acid (47.53%), while the algal oil had the highest levels of eicosapentaenoic acid (10.98%) and docosahexaenoic acid (64.73%) and the most favourable Ʃn-6/Ʃn-3 ratio ( < 0.05). All reformulated groups showed significantly improved atherogenic and thrombogenic indices and increased hypocholesterolaemic/hypercholesterolaemic ratios, which reached 17.43 in the algal oil samples ( < 0.05). Lipid oxidation was increased in the linseed and algal oil treatments, with the walnut oil group showing moderate TBARS levels and minimal accumulation of secondary oxidation products. Principal component analysis revealed that walnut oil offered the most balanced compromise between nutritional improvement, oxidative stability and sensory acceptability. These findings support a healthier reformulation of meat products by identifying oil-based fat substitutes that improve nutritional value without compromising sensory quality, which is beneficial for both research and industry.
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http://dx.doi.org/10.3390/foods14152677 | DOI Listing |
Recent Pat Biotechnol
August 2025
Department of Life Sciences, J.C. Bose University of Science and Technology, YMCA, Faridabad, Haryana-121006, India.
Introduction: The production of bio-oil from microalgae is gaining attention as an alternative renewable energy source. To generate advances in this field, it is essential to identify the gaps in existing research. Overcoming this barrier necessitates addressing methodologies that can assess the existing work and develop relationships between publications, research groups, and their impact.
View Article and Find Full Text PDFInt J Biol Macromol
September 2025
Department of Food Process Engineering, School of Bioengineering, Faculty of Engineering and Technology, SRM Institute of Science and Technology, Kattankulathur, Tamilnadu 603203, India. Electronic address:
Docosahexaenoic acid (DHA) is an omega-3 fatty acid beneficial for brain development and cardiovascular health. DHA has poor aqueous solubility and is highly susceptible to oxidation, limiting its bioavailability. To address these challenges, in the present investigation, nanoemulsion was prepared using DHA-enriched edible algal oil and tween 80 as an emulsifier (1 %) initially.
View Article and Find Full Text PDFFoods
July 2025
Institute for Animal Husbandry, Belgrade-Zemun, Autoput za Zagreb 16, 11080 Belgrade, Serbia.
The replacement of animal fat with unsaturated lipid sources in processed meats enhances nutritional value but introduces challenges regarding oxidative stability and sensory acceptability. In this study, the effects of replacing pork back fat with pre-emulsified walnut, linseed, or algal oils on the proximate composition, fatty acid profile, nutritional indices, lipid oxidation, and sensory properties of chicken frankfurters were investigated. Four formulations were prepared: a control group (25% pork fat) and three groups that were completely reformulated using oil emulsions (ratio inulin/water/oil 1:2:1).
View Article and Find Full Text PDFDiabetes Metab Syndr Obes
August 2025
Department of Internal Medicine, University of Iowa, Iowa City, IA, USA.
Objective: To answer the question what is the best source or composition of omega-3 polyunsaturated fatty acids (PUFA) that will provide the most favorable and safe outcome for peripheral neuropathy (PN) in an animal model of obesity? Traditionally encapsulated fish oil is the primary source of omega-3 PUFA as a nutritional supplement. However, other sources exist that could be a better environmental, safety, and/or economic choice.
Methods: Male Sprague Dawley rats 12 weeks of age were fed a 45% kcal diet to induce obesity and model pre-diabetes.
Food Chem
August 2025
State Key Lab of Food Science and Resources, Jiangnan University, Wuxi 214122, China; Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China. Electronic address:
Sn-2 DHA-enriched triacylglycerols (TAG) were prepared by both lipase-catalyzed esterification and solvent fractionation. The specific-structured triacylglycerol (SST) was synthesized at a molar ratio of algae oil to mixed oleic acid and linoleic acid (3:2, w/w) of 1:5 with 8 % of Lipozyme TL IM at 60 °C for 8 h. In the SSTs, the DHA content reached 46 % with sn-2 DHA/total DHA of 50 %.
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