Compact inverted digital holographic microscope based on common-path configuration.

Ongoing advancements in holographic imaging technology have a considerable impact in various fields, specifically life sciences, owing to the 3D and precise visualization of microscopic structures. However, present-day imaging technologies, such as confocal, multiphoton microscopy, and digital holography microscopy, face constraints such as tagging bulky and expensive components. Its functionality requires specific laboratory conditions and relies on relatively complicated alignment mechanics. To address these limitations, we have proposed a compact label-free, easy-to-align imaging solution, which is what we believe to be a state-of-the-art inverted holographic 3D-printed microscopy prototype based on common-path holographic configuration compatible with high numerical aperture objectives. The microscope has several advantages, including simplicity, robustness, high temporal stability, and compatibility with other imaging modalities, and it is standalone. The designed architecture is automated by using the controller and sophisticated designed stages, making it a self-focusing device along all three axes. The cutting-edge prototype presented here influences pioneering light field technology, providing simplification of the optical setup and improvement of robustness as well as real-time monitoring of live organisms and transparent samples. These features enable its applicability for in-field measurements without using an antivibration optical table. The proposed device not only overwhelms the flaws of existing technologies but also opens up new ventures for future in-field life sciences applications, to our knowledge.