Decoding metabolic trade-offs in : Chemostat-based flux remodeling for industrial ectoine biosynthesis.

, a moderately halophilic γ-proteobacterium of industrial interest, serves as a microbial cell factory for ectoine-a high-value compatible solute extensively utilized in biopharmaceuticals and cosmetics. While its ectoine biosynthesis potential is well-documented, the systemic metabolic adaptations underlying osmoadaptation remain poorly characterized, limiting rational engineering strategies for optimized production. To address this gap, we employed chemostat cultivation coupled with multi-omics integration (physiological profiling, metabolomics, and metabolic flux analysis) to dissect salt-dependent metabolic network rewiring in the model strain DSM 2581 under moderate (6.0 % NaCl) and high salinity (13.0 % NaCl) Results demonstrated that, under moderate salt conditions, a specific growth rate () of 0.20 h significantly enhanced the ectoine-specific production rate ( ), intracellular ectoine content ( ), and yield coefficient ( ), concurrent with redirection of carbon flux toward the Entner-Doudoroff (ED) pathway and ectoine biosynthesis. Under high salt conditions, flux through both the ED pathway and ectoine biosynthesis was further upregulated, whereas fluxes through the pentose phosphate (PP) pathway, tricarboxylic acid (TCA) cycle, and CO generation were downregulated. Simultaneously, suppression of the flux from malate to pyruvate enhanced oxaloacetate synthesis, thereby increasing the supply of key precursors including glutamate, aspartate, and NADPH to fuel ectoine biosynthesis. Stepwise salt reduction experiments revealed bidirectional metabolic flexibility: elevated salinity prioritized carbon investment into ED-driven ectoine production, whereas hypo-osmotic conditions reactivate respiratory activity and the TCA cycle to fuel energy metabolism. These findings establish as a paradigm of dynamic flux rewiring, where carbon economy is strategically reallocated between stress-protective solute biosynthesis and energy homeostasis. This study bridges the knowledge gap in understanding the physiological characteristics of and provides a foundation for improving ectoine production and engineering strains through metabolic optimization.