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To identify syrup adulteration in honey, a deep learning model based on the CNN-CBAM-SVM architecture combined with H NMR spectra was developed. The traditional CNN model was enhanced by incorporating the CBAM module and replacing the fully connected layer with an SVM classifier, making it well-suited for small sample sizes. The H NMR spectra of 20 genuine and 20 adulterated samples with two distinct syrups were divided into a training set (32 samples) and a validation set (8 samples). The CNN-CBAM-SVM architecture effectively addressed issues such as category imbalance in the softmax layer, weak generalization, poor robustness, and low detection accuracy typically encountered in traditional CNN models. The final model achieved 100 % accuracy on both the training and validation sets, and t-SNE visualization further confirmed the model's correct classification performance. Therefore, the integration of H NMR spectra with the CNN-CBAM-SVM model holds significant potential for detecting syrup adulteration in honey.
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http://dx.doi.org/10.1016/j.foodchem.2025.145728 | DOI Listing |
Food Chem
November 2025
College of Agriculture and Biology, Liaocheng University, Liaocheng 252059, China. Electronic address:
To identify syrup adulteration in honey, a deep learning model based on the CNN-CBAM-SVM architecture combined with H NMR spectra was developed. The traditional CNN model was enhanced by incorporating the CBAM module and replacing the fully connected layer with an SVM classifier, making it well-suited for small sample sizes. The H NMR spectra of 20 genuine and 20 adulterated samples with two distinct syrups were divided into a training set (32 samples) and a validation set (8 samples).
View Article and Find Full Text PDFInsects
June 2025
Department of Biochemistry, Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbia.
The enzymes in honey can originate not only from bees and the plants from which the bees collect pollen and nectar but also from feed provided by beekeepers. Enzymes that hydrolyze sucrose-present in honey (α-glucosidase) or honey adulterated with invert syrup (β-fructofuranosidase)-can be distinguished using zymography, where enzymatic bands are detected with nitroblue tetrazolium (NBT) after sugar removal via ultrafiltration. This method enables the identification of honey produced in hives that have been improperly fed with invert syrup, leading to the mixture of natural honey and syrup, and offers a practical tool to detect indirect adulteration.
View Article and Find Full Text PDFJ Appl Glycosci (1999)
May 2025
1 Japan Food Research Laboratories.
Western honeybee () α-glucosidase III (HBG-III), which is secreted from the hypopharyngeal glands of honeybees, plays a role in converting nectar into honey. Consequently, hypothesizing that HBG-III is a suitable marker of honey authenticity, we developed an analytical method to determine the HBG-III content and investigated its applicability to various commercial products. Following extraction from honey using phosphate-buffered saline, HBG-III was concentrated using an ultrafiltration membrane and subsequently fragmented with trypsin and lysyl endopeptidase mixture.
View Article and Find Full Text PDFFood Chem
September 2025
State Key Laboratory of Resource Insects, Institute of Apiculture Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China. Electronic address:
Honey contains abundant oligosaccharides which play an important role in controlling honey quality. However, there have been few reports on their formation mechanism in honey. In this study, the formation mechanism of turanose and erlose was proposed by analyzing sugar composition of obtained honey samples combined with C labeled sucrose experiments.
View Article and Find Full Text PDFFood Chem
September 2025
College of Agriculture and Biology, Liaocheng University, Liaocheng 252059, China. Electronic address:
To determine the authenticity of honey, a deep learning network based on the Canny-GoogLeNet architecture combined with three-dimensional (3D) fluorescence spectroscopy was established. The canny edge detection algorithm was used to extract 3D spectral data from two distinct monofloral honeys, rape honey and wolfberry honey, as well as adulterated honey samples with corn syrup or other types of honey. The dataset was divided into training (133 samples), validation (33 samples), and test sets (12 samples).
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