Spatial metabolic heterogeneity in (Schw.) Wolf (): Insights from quantitative analysis and widely targeted metabolomics.

Food Chem X

State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijng 100700, China.

Published: July 2025


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Article Abstract

(Schw.) Wolf () is valued for its nutritional and therapeutic properties. is commonly processed into slices of different shapes and sizes. We quantified alkali-soluble and water-soluble polysaccharides, total amino acids, and triterpenoids in slices of varying geometries (square: 1-9 cm width; round: 1-6 cm radii). Results showed that triterpenes and water-soluble polysaccharides initially decreased and then stabilized with increasing slice size, while total amino acids accumulated progressively, indicating spatial metabolic heterogeneity in . To map metabolite distribution, we segmented the into seven concentric zones and profiled the metabolites using a widely targeted metabolomics approach. A total of 359 metabolites were identified. The fungal symbiotic matrix exhibited metabolic profiles similar to the intermediate regions, while peripheral regions maintained comparable levels of triterpenes, saccharides, and amino acid derivatives. This study provides a detailed metabolomic blueprint of and offers insights for standardized processing and optimized medicinal use.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12304756PMC
http://dx.doi.org/10.1016/j.fochx.2025.102802DOI Listing

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