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Objectives: Endodontic retreatment aims to address treatment failure through the removal of root canal filling materials. This in vitro study evaluated the presence of filling material remnants in the mesial root canals, specifically focusing on the isthmuses, of mandibular molars after retreatment.
Methods: One hundred extracted mandibular molar mesial roots with isthmuses were prepared with an R25 file, obturated with one of five calcium silicate-based sealers (BioRoot RCS [Septodont], MTApex [Ultradent Products Inc.], EndoSequence BC Sealer HiFlow [Brasseler USA], Bio-C Sealer [Angelus]) or an epoxy resin-based sealer (AH Plus Jet [Dentsply Maillefer]), all stained with rhodamine B, and stored at 37ºC for 30 days to allow for setting. Retreatment was subsequently performed using R40 and XP-endo Finisher R instruments (FKG Dentaire) with 2.5% sodium hypochlorite irrigation. The presence of remaining filling material was then assessed using confocal microscopy, and setting times were tested per ISO 6876:2012.
Results: AH Plus Jet showed the most remnants at 2 mm and the longest retreatment time. Calcium silicate-based sealers exhibited prolonged setting times under dry conditions, with EndoSequence BC Sealer HiFlow showing a particularly extended setting period.
Conclusions: Despite retreatment, residues remained in all canals and isthmus regions, particularly Bio-C Sealer and AH Plus Jet in apical areas, emphasizing the difficulty of complete removal and the persistence of filling material.
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http://dx.doi.org/10.5395/rde.2025.50.e25 | DOI Listing |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12415234 | PMC |
Restor Dent Endod
August 2025
Department of Endodontics, School of Dentistry, Grande Rio University (UNIGRANRIO), Rio de Janeiro, RJ, Brazil.
Objectives: This study aimed to assess the influence of two endodontic sealers on the bond strength of glass fiber posts using conventional and self-adhesive resin cement through a push-out test.
Methods: Forty central human incisors were randomly divided into four groups (n = 10) based on sealer (epoxy resin- based or calcium silicate-based) and cement (conventional and self-adhesive resin) types: AH Plus (Dentsply De- Trey)/RelyX ARC (3M ESPE), AH Plus/RelyX U200 (3M ESPE), Bio-C Sealer (Angelus)/RelyX ARC, and Bio-C Sealer/RelyX U200. After canal filling and post cementation, roots were sectioned to obtain one specimen per root third.
Aust Endod J
September 2025
Department of Endodontics, Faculty of Dentistry, Oral and Dental Diseases Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
This study aimed to evaluate the biocompatibility of newly synthesised cement (Nanodentin) in comparison with Biodentine and CEM cement. The cements were implanted in rat skin and tissue reactions were assessed after 7, 30 and 60 days. H&E staining was used to measure the inflammatory response, the number of inflammatory cells and the presence of necrosis.
View Article and Find Full Text PDFDent Mater
September 2025
Faculty of Dentistry, National University of Singapore, Singapore; ORCHIDS: Oral Care Health Innovations and Designs Singapore, National University of Singapore, Singapore. Electronic address:
Objectives: To develop and validate predictive machine learning model capable of estimating long-term pH profiles (up to 672 h) of calcium silicate-based cements (CSCs) using early-stage pH measurements (3 and 24 h).
Materials And Methods: pH and calcium ion release data from in vitro studies (2014 - 2024) were extracted and analysed using descriptive statistics and correlation metrics. Feature selection was conducted using Random Forest regressors to identify key variables.
Int Dent J
September 2025
Discipline of Endodontology, Division of Restorative Dental Sciences, Faculty of Dentistry, The University of Hong Kong, Hong Kong SAR, China. Electronic address:
Objectives: This study aimed to evaluate the cytotoxicity and osteogenic potential of three sealers, including a strontium silicate-based sealer, C-Root SP (C-R), and two calcium silicate-based sealers, iRoot SP (i-R) and AH Plus Bioceramic Sealer (AHPbcs), compared with AH Plus (AHP) on MC3T3-E1 pre-osteoblasts.
Materials And Methods: Standardized sealer discs were eluted in a culture medium to assess cytotoxicity using the CCK-8 assay at various dilutions (1:1, 1:2, 1:5, and 1:10). Osteogenic differentiation was evaluated by culturing cells in osteogenic medium supplemented with 1:5 diluted sealer extract.
Cureus
July 2025
Department of Conservative Dentistry and Endodontics, Kalinga Institute of Dental Sciences, Bhubaneswar, IND.
Regenerative endodontics utilizes stem cell biology and bioactive materials to restore pulp vitality. Human dental pulp stem cells (hDPSCs), with their self-renewal and odontogenic differentiation potential, are central to regenerative endodontics. Hydraulic calcium silicate-based cements (HCSCs), such as mineral trioxide aggregate (MTA) and Biodentine (Septodont, Saint-Maur-des-Fossés, France), are widely used in vital pulp therapies to promote pulp vitality recovery.
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