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Article Abstract
Background: (), a significant pathogen in gastrointestinal diseases, has been implicated in various oral pathologies. Accurate detection of in oral biopsies is crucial for understanding its role in oral diseases.
Materials And Methods: A total of 100 oral biopsy samples were collected from patients presenting with suspected oral lesions. The samples were subjected to Giemsa staining and immunohistochemistry (IHC) using anti- antibodies. The sensitivity, specificity, and diagnostic accuracy of Giemsa staining were assessed using IHC as the gold standard. Arbitrary values: Giemsa-positive cases were observed in 65% of samples, while IHC detected in 70%. Statistical analysis was conducted using Chi-square tests and kappa agreement.
Results: Giemsa staining exhibited a sensitivity of 85%, specificity of 90%, and an overall diagnostic accuracy of 88% when compared to IHC. There was substantial agreement (kappa = 0.76) between the two methods. While Giemsa staining was less expensive and quicker, IHC provided greater specificity, particularly in cases with low bacterial density.
Conclusion: Giemsa staining is a reliable, cost-effective method for detecting in oral biopsy specimens. However, IHC remains the gold standard due to its higher specificity and ability to detect low-density infections. Combining both techniques may enhance diagnostic accuracy in clinical settings.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12244783 | PMC |
| http://dx.doi.org/10.4103/jpbs.jpbs_127_25 | DOI Listing |
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