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Strain 3B1 was isolated from the soil of rice field cultivated under the system of rice intensification (SRI) in Sukabumi, West Java, Indonesia. The genome of strain 3B1 was sequenced using the MGI DNBSEQ platform, followed by bioinformatics processing, including genome assembly and gene annotation using SPAdes and Prokka, respectively. The assembled genome had a total length of 5,137,985 bp, distributed across 70 contigs, with 5,364 genes identified. Strain 3B1 shared the highest 16S rRNA gene sequence identity including , and , in the range of 99.86 to 99.93%. Both 16S rRNA gene and core genes-based phylogenetic analyses placed strain 3B1 in the same clade with strain Ames within the genus. The phylogenetic placement was supported by the highest average nucleotide identity (ANI) value of 98.1% and digital DNA-DNA hybridization (dDDH) value of 82.7% shared between the genomes of strain Ames and strain 3B1, indicating that 3B1 is a strain of . Further gene annotation revealed that the genome of strain 3B1 lacked the genes encoding for virulence factors such as the , and . Nonetheless, this data provides valuable insights into the genomic feature of strain 3B1, which can be bioprospected for various biotechnological applications.
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http://dx.doi.org/10.1016/j.dib.2025.111586 | DOI Listing |
Sci Rep
July 2025
Department of Medical and Surgical Sciences (DIMEC), University of Bologna, Bologna, 40138, Italy.
Recent Monkeypox virus (MPXV) outbreaks in non-endemic regions have highlighted the need for genomic surveillance to support epidemiological investigations and monitor viral evolution. In this paper we present the results of genomic characterization and analyses of mechanisms of human adaptation, including APOBEC-style mutations, performed on 11 MPXV isolates, collected from May to September 2022, from Emilia-Romagna (Italy). Phylogenetic analysis confirmed all strains belonged to Clade IIb.
View Article and Find Full Text PDFData Brief
June 2025
Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, West Java, Indonesia.
Strain 3B1 was isolated from the soil of rice field cultivated under the system of rice intensification (SRI) in Sukabumi, West Java, Indonesia. The genome of strain 3B1 was sequenced using the MGI DNBSEQ platform, followed by bioinformatics processing, including genome assembly and gene annotation using SPAdes and Prokka, respectively. The assembled genome had a total length of 5,137,985 bp, distributed across 70 contigs, with 5,364 genes identified.
View Article and Find Full Text PDFCell
March 2025
Department of Agricultural, Food & Nutritional Science, University of Alberta, Edmonton, AB T6G 2E1, Canada; APC Microbiome Ireland, University College Cork, Cork T12 YT20, Munster, Ireland; School of Microbiology, University College Cork, Cork T12 YT20, Munster, Ireland; Department of Medicine, Uni
Industrialization adversely affects the gut microbiome and predisposes individuals to chronic non-communicable diseases. We tested a microbiome restoration strategy comprising a diet that recapitulated key characteristics of non-industrialized dietary patterns (restore diet) and a bacterium rarely found in industrialized microbiomes (Limosilactobacillus reuteri) in a randomized controlled feeding trial in healthy Canadian adults. The restore diet, despite reducing gut microbiome diversity, enhanced the persistence of L.
View Article and Find Full Text PDFHelicobacter
October 2023
Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, China.
Background: It has been documented that Helicobacter hepaticus produces a nickel-containing hydrogen-oxidizing hydrogenase enzyme, which is necessary for hydrogen-supported amino acid uptake. Although H. hepaticus infection has been shown to promote liver inflammation and fibrosis in BALB/c mice, the impact of hydrogenase on the progression of liver fibrosis induced by H.
View Article and Find Full Text PDFInt J Surg Open
June 2023
Division of Pathology, ICAR-Indian Veterinary Research Institute, Bareilly, Uttar Pradesh, 243122, India.
The highly contagious Omicron variant of SARS-CoV-2 is a recent cause of concern during the COVID-19 pandemic. The World Health Organization (WHO) has classified SARS-CoV-2 variants into variants of concern (VOCs), variants of interest (VOIs), and variants under monitoring (VUMs). VOCs were categorized as Alpha (B.
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