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The use of enriched rotifers with industrially produced microalgae represents a valuable tool for the enhancement of zebrafish larval nutrition and increased biological performance. Currently, a monoculture of microalgal species ( sp.) in form of liquid paste is routinely used for rotifers enrichment for zebrafish larvae feeding; however, the most adequate typology (i.e., paste or freeze-dried) of the industrially produced microalgal biomass is still controversial. This work aimed to compare the effects of rotifers enriched with three different industrially produced microalgae species (i.e., , , and ) using paste and freeze-dried powder. Enriched rotifers were provided as feed during larval growth and the impact on growth and survival was evaluated. The use of enriched rotifers with both paste or freeze-dried microalgae improved growth compared to larvae fed exclusively with commercial microdiet. Larvae fed rotifers enriched with and attained higher weight and length both at 15 and 30 days postfertilization (dpf), while the use of microalgae in paste contributed to greater larvae lengths when compared to freeze-dried. The experimental results in this study revealed that and in paste are the most suitable microalgae forms to be used in zebrafish larvae nutrition and in the improvement of enrichment methodologies for rotifers.
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http://dx.doi.org/10.1155/anu/5351038 | DOI Listing |
Toxicol Sci
September 2025
Aquatic and Crop Resource Development, National Research Council of Canada, Halifax, NS, B3H 3Z1, Canada.
In the zebrafish larval toxicity model, phenotypic changes induced by chemical exposure can potentially be explained and predicted by the analysis of gene expression changes at sub-phenotypic concentrations. The increase in knowledge of gene pathway-specific effects arising from the zebrafish transcriptomic model has the potential to enhance the role of the larval zebrafish as a component of Integrated Approaches to Testing and Assessment (IATA). In this paper, we compared the transcriptomic responses of triphenyl phosphate between two standard exposure paradigms, the Zebrafish Embryo Toxicity (ZET) and General and Behavioural Toxicity (GBT) assays.
View Article and Find Full Text PDFComp Biochem Physiol C Toxicol Pharmacol
September 2025
Department of Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu, India. Electronic address:
Excessive fluoride (F) exposure, particularly during early development, poses a significant risk to skeletal integrity by disrupting bone homeostasis through oxidative stress and altered mineralization. While F induced oxidative stress is well documented, studies investigating the role of natural antioxidants in mitigating F induced osteochondral toxicity remains limited. Hence, the present study investigated the osteomodulatory effect of fisetin (Fis) against F toxicity in zebrafish larvae.
View Article and Find Full Text PDFPLoS Pathog
September 2025
State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Hepatocyte nuclear factor 4 alpha (Hnf4α), a conserved nuclear receptor central to vertebrate liver development and metabolic regulation, emerges here as a pivotal immune regulator in teleosts against complex infectious threats. While its metabolic roles are well-established, Hnf4α's function in bacterial infection, viral infection, and bacterial-viral coinfection-major challenges in global aquaculture-remained uncharacterized. This study reveals that teleost Hnf4α acts as a dual-functional immune checkpoint, essential for combating Aeromonas salmonicida, grass carp reovirus (GCRV), and their coinfection.
View Article and Find Full Text PDFJ Vis Exp
August 2025
School of Life Sciences, Shanghai University; Division of Biology and Biological Engineering, California Institute of Technology;
The hypothalamus is an ancient brain region that regulates diverse aspects of physiology and behavior, including sleep and wakefulness, appetite, energy homeostasis, anxiety, depression, and social interaction. Specific neuronal populations in the hypothalamus exert their effects via the release of neurotransmitters and neuropeptides. Whole-cell patch-clamp recording is an indispensable approach for studying the roles of these factors in synaptic transmission and brain function.
View Article and Find Full Text PDFDrug Dev Res
September 2025
Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug/Gene Delivery and Tissue Engineering, Jiangsu Provincial Research Center for Medicinal Function Development of New Food Resources, Jiangsu University, Zhenjiang, Jiangsu, China.
Liver cancer is the fourth most deadly cancer worldwide, but existing treatment options are insufficient, thus highlighting the urgent need for new therapeutic agents. Taxanes, known for their anticancer properties, provide a promising avenue for intervention. In this study, a tetracyclic taxane compound with antitumor activity (taxinine) was extracted and isolated from Taxus chinensis (T.
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