Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The proper function of lysosomes depends on their ability to store and release calcium. While several lysosomal calcium release channels have been described, how lysosomes replenish their calcium stores in placental mammals has not been determined. Using genetic depletion and overexpression techniques combined with electrophysiology and visualization of subcellular ion concentrations and their fluxes across the lysosomal membrane, we show here that TMEM165 imports calcium to the lysosomal lumen and mediates calcium-induced lysosomal proton leakage. Accordingly, TMEM165 accelerates the recovery of cells from cytosolic calcium overload thereby enhancing cell survival while causing a significant acidification of the cytosol. These data indicate that in addition to its previously identified role in the glycosylation of proteins and lipids in the Golgi, a fraction of TMEM165 localizes on the lysosomal limiting membrane, where its putative calcium/proton antiporter activity plays an essential role in the regulation of intracellular ion homeostasis and cell survival.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12141690 | PMC |
| http://dx.doi.org/10.1038/s41467-025-60349-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The flavonoid rutin protects against imidacloprid-induced osmotic and electric disruptions in Africanized honey bees.
PLoS One
September 2025
Departamento de Biología, Escuela de Ciencias e Ingeniería, Universidad del Rosario, Bogotá, Colombia.
Honey bees (Apis mellifera) are essential pollinators threatened by sublethal effects of pesticides such as imidacloprid, a widely used neonicotinoid that disrupts the central nervous system. However, many of the systemic effects are poorly understood, especially on the physiological homeostasis of the honey bee. We evaluated the effects of oral administration of imidacloprid and the flavonol rutin on the properties of extracellular fluid (ECF) in Apis mellifera.
View Article and Find Full Text PDFSodium Orthovanadate (SOV) mitigates alcohol & alcohol plus high-fat diet (HFD)-induced hepatotoxicity in rats.
Cell Mol Biol (Noisy-le-grand)
September 2025
Associate Professor, School of Pharmacy, Desh Bhagat University, Mandi Gobindgarh-Punjab 147301, India.
Alcoholic fatty liver disease (AFLD) is a leading cause of chronic liver disease worldwide, contributing to significant morbidity and mortality. Despite its growing prevalence, no FDA-approved pharmacological treatments exist, leaving lifestyle modifications as the primary intervention. AFLD pathogenesis involves a complex interplay of lipid accumulation, oxidative stress, insulin resistance, and inflammation, highlighting the need for innovative therapeutic approaches.
View Article and Find Full Text PDFCharacterization and expression analysis of transcription factors in unveil their critical roles in salt stress resistance.
Front Plant Sci
August 2025
Nanfan Research Institute, Chinese Academy of Agricultural Sciences (CAAS), Sanya, Hainan, China.
Introduction: Transcription factors (TFs) are essential regulators of gene expression, orchestrating plant growth, development, and responses to environmental stress. , a halophytic species renowned for its exceptional salt resistance, provides an ideal model for investigating the regulatory mechanisms underlying salt tolerance.
Methods: Here, we present a comprehensive genome-wide identification and characterization of TFs in .
The Role of Phospholipids in Mitochondrial Dynamics and Associated Diseases.
Front Biosci (Landmark Ed)
August 2025
University of Angers, MitoLab, Unité MITOVASC, UMR CNRS 6015, INSERM U1083, SFR ICAT, 49330 Angers, France.
The bioenergetic machinery of the cell is protected and structured within two layers of mitochondrial membranes. The mitochondrial inner membrane is extremely rich in proteins, including respiratory chain complexes, substrate transport proteins, ion exchangers, and structural fusion proteins. These proteins participate directly or indirectly in shaping the membrane's curvature and facilitating its folding, as well as promoting the formation of nanotubes, and proton-rich pockets known as cristae.
View Article and Find Full Text PDFEnvironmental stressor-induced functional and expression dynamics of glutathione S-transferase genes in bees.
Pestic Biochem Physiol
November 2025
College of Life Sciences, Chongqing Normal University, Chongqing, China; Key Laboratory of Pollinator Resources Conservation and Utilization of the Upper Yangtze River, Ministry of Agriculture and Rural Affairs, Chongqing, China; Chongqing Key Laboratory of Vector Control and Utilization, Chongqing,
As key pollinators, bees are increasingly threatened by environmental stressors such as heavy metals, pesticides, and temperature fluctuations, which can cause oxidative stress and disrupt cellular homeostasis. Glutathione S-transferases (GSTs) play crucial roles in antioxidant defense and detoxification, yet systematic studies on bee GST families remain limited. Here, we conducted a genome-wide analysis of cytosolic GST genes in 13 bee species, identifying 146 genes in total.
View Article and Find Full Text PDF