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Context: The assessment of reestablishment of correct anatomic contour and proximal tightness with minimal gingival overhang of a class II restoration.
Aim: This study evaluated and compared the gingival overhang, contact, and contour anatomy achieved by four matrix systems for class II restorations.
Materials And Method: Standardized class II mesio-occlusal conservative cavities were prepared on two hundred typodont teeth and assigned into four groups ( = 50): Tofflemire, U Band system, Palodent V3 matrix system, and Walser Matrix system. The cavities were restored with composite and qualitatively analyzed for proximal contact tightness. The morphology of the overhang and contour was assessed using a Medit scanner and Mesh Labs by superimposition.
Statistical Analysis Used: Kruskal-Wallis nonparametric test of significance and Mann-Whiteny -test for pair-wise comparison was used.
Results: The restorations showed statistically significant difference, with Walser matrix system and U Band demonstrating minimal overhang. No significant difference was observed for contact tightness among the groups ( = 0.109). Contours were assessed at the occlusal, middle, and cervical thirds. The Walser matrix system provided the best gingival contour ( = 0.000). The Palodent V3 matrix yielded the best contour at the middle ( = 0.002) and occlusal ( = 0.036) third with minimum deviation from optimal contour.
Conclusions: The sectional precontoured matrix system Palodent V3 system recreated the best proximal contour and contact anatomy, while minimal overhang was generated by the Walser matrix.
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http://dx.doi.org/10.4103/JCDE.JCDE_143_25 | DOI Listing |
Nutr J
September 2025
Department of Life Sciences, Division of Food and Nutrition Science, Chalmers University of Technology, Gothenburg, 412 96, Sweden.
Background: Avenanthramides (AVAs) and Avenacosides (AVEs) are unique to oats (Avena Sativa) and may serve as biomarkers of oat intake. However, information regarding their validity as food intake biomarkers is missing. We aimed to investigate critical validation parameters such as half-lives, dose-response, matrix effects, relative bioavailability under single dose, and in relation to the abundance of Feacalibacterium prausnitzii, and under repeated dosing, to understand the potential applications of AVAs and AVEs as biomarkers of oat intake.
View Article and Find Full Text PDFNat Metab
September 2025
Cellular and Molecular Physiology Department, Yale School of Medicine, New Haven, CT, USA.
The essential cofactor coenzyme A (CoASH) and its thioester derivatives (acyl-CoAs) have pivotal roles in cellular metabolism. However, the mechanism by which different acyl-CoAs are accurately partitioned into different subcellular compartments to support site-specific reactions, and the physiological impact of such compartmentalization, remain poorly understood. Here, we report an optimized liquid chromatography-mass spectrometry-based pan-chain acyl-CoA extraction and profiling method that enables a robust detection of 33 cellular and 23 mitochondrial acyl-CoAs from cultured human cells.
View Article and Find Full Text PDFGenes Immun
September 2025
Department of Genetics, University Medical Center Groningen, University of Groningen, Groningen, The Netherlands.
In coeliac disease (CeD), the epithelial lining (EL) of the small intestine is severely damaged by a complex auto-inflammatory response, leading intraepithelial lymphocytes to attack epithelial cells. To understand the intestinal changes and genetic regulation in CeD, we investigated the heterogeneity in the transcriptomic profile of the duodenal EL using RNA-seq and eQTL analysis on predicted cell types. The study included duodenal biopsies from 82 patients, grouped into controls, gluten-free diet treated CeD and untreated CeD.
View Article and Find Full Text PDFInt J Pharm
September 2025
Laboratory of Advanced Theranostic Materials and Technology, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, PR China; Zhejiang International Scientific and Technological Cooperative Base of Biomedical Materials and Technology, Ningbo Cixi Instit
Smart hydrogels have advanced rapidly in recent years. However, systems responsive to a single stimulus are typically triggered by specific cues, limiting their adaptability in complex and dynamic biological environments. To overcome this limitation, this study developed a dual-responsive hydrogel sensitive to both temperature and mechanical stress.
View Article and Find Full Text PDFMar Environ Res
September 2025
Division of Earth and Environmental System Sciences, Pukyong National University, Busan, 48513, Republic of Korea. Electronic address:
A total of 27 Alexandrium catenella strains isolated from Jinhae-Masan Bay were examined to assess differences in the toxicity and composition of paralytic shellfish toxins (PST). The strains exhibited widely variable toxicity, ranging from 0.02 to 360.
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