Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
The cell membrane of lactic acid bacteria (LAB) functions as a crucial barrier against external conditions. Spray drying, a technique used for large-scale bacterial powder production, exposes cells to high temperatures, resulting in membrane damage. In this study, three strains of A12, F1-7, and KV9 with significant differences in survival rates after spray drying (47.28 ± 9.00, 11.12 ± 2.62, and 37.44 ± 0.22%, respectively) were selected for analysis. A12 exhibited the strongest SYTO-9 fluorescence intensity, indicating the highest membrane integrity after spray drying. Subsequently, UHPLC-Q-Exactive MS-based lipidomics identified two key lipid species, fatty acyl 18:1; O3 and cardiolipin 15:0_18:0_28:0_22:5, which were potentially crucial for bacterial membrane heat resistance. KEGG pathway analysis further revealed that glycerophospholipid metabolism was the most significantly enriched pathway. This study provides insights into critical membrane lipids that contribute to the heat resistance of during spray drying, offering a theoretical reference for screening stress-resistant LAB.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/acs.jafc.5c02351 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
"Engineering Rifampicin-Encapsulated Inhalable Microparticles for Precision Tuberculosis Therapy: In Vivo Distribution and Therapeutic Evaluation".
J Drug Target
September 2025
Department of Pharmaceutics, ShriRam College of Pharmacy, Banmore, Morena-476444, Madhya Pradesh, India.
Tuberculosis (TB) continues to cause significant global mortality, highlighting the need for improved drug delivery systems. The objective of this paper focuses in describing the formulation, optimization and in vivo assessment of rifampicin encapsulated PLGA microparticles for site-specific inhalation therapy. Microparticles for inhalation were produced by spray drying, and the DoE methodology was applied to reach the most suitable aerodynamic properties (mass median aerodynamics diameter (MMAD) 2.
View Article and Find Full Text PDFDesign, Development and Evaluation of Orally Disintegrating Mini-tablets (ODMTs) Containing Cefixime for Paediatric Use: A Novel Approach.
Turk J Pharm Sci
September 2025
Drugs Testing Laboratory, Department of Drugs Control, Bangalore, India.
Objectives: The study aimed to combine instant-release and mini-tablet methodologies to develop novel orally disintegrating mini-tablets (ODMTs) for a frequently pescribed antibiotic, cefixime trihydrate (CT), in paediatric patients.
Materials And Methods: CT-loaded microcapsules were prepared using Eudragit EPO and Hydroxy Propyl Methyl Cellulose E50 by spray drying technique. The optimized microcapsules were mixed with co-processed ready-to-use tableting excipients, Ludiflash and Pearlitol 200SD, in different proportions and then compressed into ODMTs and evaluated.
A Supraparticle-Based Approach to Robust Biomimetic Superhydrophobic Coatings.
Small
September 2025
Institute of Interfaces and Particle Technology, Friedrich-Alexander-Universität Erlangen-Nürnberg, Cauerstrasse 4, 91058, Erlangen, Germany.
Repellent surfaces provide resistance to biofouling, ice formation, bacteria adhesion, or corrosion. Inspired by the hierarchical structure of the lotus leaf, such surfaces minimize water adhesion through micro- and nanostructuring. Conventional fabrication methods to mimic the lotus leaf often involve problematic fluorinated compounds, sophisticated preparation conditions, or lack mechanical robustness.
View Article and Find Full Text PDFSpray film-forming system formulation of as a diabetic wound healer.
J Adv Pharm Technol Res
August 2025
Faculty of Public Health Universitas Ahmad Dahlan, Yogyakarta, Indonesia.
Diabetic wounds are chronic complications in patients with diabetes mellitus characterized by an elongated proliferation period, leading to a longer wound closure time. The lack of topical remedies for diabetic wounds necessitates the development of formulations that effectively facilitate closure. Kratom () leaves, containing polyphenolic compounds, have the potential to be developed into a film spray suitable for diabetic wound closure.
View Article and Find Full Text PDFPowdered camel milk production and changes occurring along the processing steps and storage conditions: A comprehensive review.
Food Chem
August 2025
Department of Chemical and Petroleum Engineering, Khalifa University, Abu Dhabi, United Arab Emirates. Electronic address:
Spray drying offers high-quality camel milk powder with a good nutritional and techno-functional profile. However, the thermal stress can cause whey protein denaturation, reducing the effectiveness of the bioactive components of the milk and compromising its health benefits. Camel milk caseins are more sensitive to heat, while its whey proteins exhibit greater resistance compared to those in bovine milk.
View Article and Find Full Text PDF