Tree swallow (Tachycineta bicolor) demographics and metal(loid) concentrations in egg contents from the Kootenai River basin, Montana nest box colonies.

Selenium (Se) levels in water have been increasing in Lake Koocanusa and the Kootenai River below Libby Dam in Montana due to coal mining in the drainage basin of the Elk River, British Columbia. Aquatic monitoring of Se is ongoing to assess potential effects; however, exposure to terrestrial, aquatic-dependent wildlife has not been investigated. Tree swallows are a targeted receptor for evaluation of potential Se-related effects as this species is a mid-level consumer with aquatic-terrestrial food web linkages, is sensitive to Se, and reliably uses nest boxes. The goals of this project were to identify tree swallow demographics and metal(loid) concentrations to assess potential species and ecosystem-level effects within the Kootenai River basin study area. During 2022 and 2023, a total of 98 nest boxes were monitored across ~ 120 km of shoreline; 60 eggs were collected. Egg metal(loid) concentrations, including Se, were below levels identified to cause reproductive effects in avian species. However, nest box occupancy increased with distance from the US/Canada border (P < 0.001; R = 0.83), and Se concentrations were significantly higher (P = 0.014) in 2023 than 2022 (3.2 (3.0-3.4) and 2.8 (2.6-3.1), respectively; geometric mean, 95% CI; mg/kg, dw). With ongoing mining operations in the drainage basin of the Elk River, these results suggest that long-term monitoring of tree swallows will support assessment of spatiotemporal metal(loid) trends. Additional data, including tree swallow foraging patterns, will allow evaluation of differences in nest box occupancy and metal(loid) concentrations in the Kootenai River basin.