Effect of linseed oil and oleogels on the thermal aggregation behavior of myofibrillar protein from Nemipterus virgatus.

The impact of 1 % linseed oil or 1.08 % oleogels prepared using γ-oryzanol and β-sitosterol on the thermal aggregation behavior of myofibrillar protein (MP) was explored. The results showed that the turbidity and particle size of MP solution significantly increased after heating for 40 min. The addition of linseed oil enhanced the whiteness, turbidity and sulfhydryl content of MP, but decreased the hydrophobic interactions and disulfide bond content. When oleogels were added, the MP structure unfolded more readily during heating, and the turbidity, active sulfhydryl content and surface hydrophobicity increased by 7.80 %, 13.96 % and 60.33 % after 50 min, respectively. Oleogels reduced the fluorescence intensity of MP and contributed to the conformational transformation from α-helix to β-sheet structure. The protein aggregates in oleogels group were relatively uniform and regular in dispersion. Therefore, oleogels enhanced the unfolding of MP, and improved the thermal aggregation behavior of MP during heating.