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Introduction: Anthocyanins are crucial secondary metabolites that are responsible for pigment deposition in fruits. Raspberry fruit color shifts from white to red during natural or postharvest ripening. However, the precise mechanisms and biosynthetic pathways of anthocyanins in postharvest raspberries remain unclear.
Methods: This study used metabolomic and transcriptomic analyses to explore anthocyanin biosynthesis in postharvest raspberries at various color stages: white (RBT-1), white-to-pink (RBT-2), pink (RBT-3), red (RBT-4), and deep red (RBT-5).
Results: We identified 43 key metabolites, and 13,239 DEGs linked to anthocyanin biosynthesis in postharvest raspberry colour development, including cyanidin-3-O-sophoroside and cyanidin-3-O-glucoside. The key DAMs in colored raspberries were gentiobioside, pelargonidin-3,5-O-diglucoside, cyanidin-3-O-sambubioside, and pelargonidin-3-O-sambubioside. Transcriptome analysis revealed 32 differentially expressed structural genes linked to anthocyanin and flavonoid synthesis, with significant upregulation of , and genes, which promote anthocyanin synthesis and pigment accumulation. Integrated analysis showed that cyanidin-3-O-sophoroside was correlated with 9 structural genes involved in anthocyanin biosynthesis, 19 transcription factors (TFs), and 14 hormone signaling-related genes.
Discussion: This study explored the regulatory mechanisms of MYB, WRKY, bHLH, and NAC transcription factors, as well as structural genes and phytohormone-related genes, in modulating anthocyanin metabolism during postharvest color changes in raspberries. The findings provide valuable insights for optimizing postharvest fruit storage conditions and enhancing fruit quality.
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http://dx.doi.org/10.3389/fpls.2025.1549458 | DOI Listing |
Nutr Rev
September 2025
Department of Pharmaceutics, Manipal College of Pharmaceutical Sciences, Manipal Academy of Higher Education, Manipal, Karnataka 576104, India.
Pomegranate (Punica granatum L) is a rich source of bioactive compounds, including punicalagin, ellagic acid, anthocyanins, and urolithins, which contribute to its broad pharmacological potential. This review summarizes evidence from in vitro and in vivo experiments, as well as clinical studies, highlighting pomegranate's therapeutic effects in inflammation, metabolic disorders, cancer, cardiovascular disease, neurodegeneration, microbial infections, and skin conditions. Mechanistic insights show modulation of pathways such as nuclear factor-kappa B (NF-κB), mitogen-activated protein kinase (MAPK), alpha serine/threonine-protein kinase (AKT1), and nuclear factor erythroid 2-related factor 2 (Nrf2).
View Article and Find Full Text PDFFood Res Int
November 2025
Innovative Technology, Food and Health Research Group, Facultad de Industrias Alimentarias, Universidad Nacional Agraria La Molina, Av. La Molina s/n, La Molina, Lima, Peru; Innovative Technology, Food and Health Research Group, Instituto de Investigación de Bioquímica y Biología Molecular, Unive
Tea is consumed worldwide, and it is highly appreciated by consumers as a functional, healthy, and natural drink. The objectives of this research were to evaluate (1) the storage stability and (2) the consumption effect on biomarkers of oxidative stress of an antioxidant tea prepared from purple corn cob and stevia (AOxTea). The AOxTea bags were subjected to storage environments of 75 or 85 % of relative humidity at 30, 40 and 50 °C for up to 19 days.
View Article and Find Full Text PDFFood Res Int
November 2025
State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, Liaoning, China; Academy of Food Interdisciplinary Science, School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, Liaoning, China; National Engineering Rese
Anthocyanins (AC) are natural bioactive substances with the excellent antioxidant properties, but its structure is susceptible to the external environmental factors with inevitably decreased bioavailability. In this work, γ-cyclodextrin based metal-organic framework (CD-MOF) shows high encapsulation efficiency (96.09 %) and satisfiable loading amount (24.
View Article and Find Full Text PDFFront Plant Sci
August 2025
Department of Plant Science and Landscape Architecture, University of Maryland, College Park, MD, United States.
Fruit drop, cracking, and advanced ripening prior to fruit harvest can promote significant losses in important apple cultivars such as 'Ambrosia' and 'Fuji' grown in the mid-Atlantic. These losses result from environmental factors, delays in harvest due to the lack of red skin color development, and cultivar-specific characteristics, among others. Aminoethoxyvinylglycine (AVG) and 1-methylcyclopropene (1-MCP) are ethylene-inhibiting plant growth regulators (PGRs) that can alter preharvest fruit drop, cracking, maturity, and quality by impeding ethylene biosynthesis and perception, respectively.
View Article and Find Full Text PDF, commonly known as sweet potato, is an increasingly valued functional food because of its vivid coloration and rich bioactive compounds, especially anthocyanins and carotenoids, such as ipomoeaxanthin. This review focuses on the bioavailability, mechanisms of action, and therapeutic potential of sweet potato-derived anthocyanins in diabetes and metabolic disorders. Anthocyanins, which are plant pigments, exhibit high antioxidant activity by scavenging free radicals and stimulating endogenous antioxidant enzymes such as catalase and superoxide dismutase, thereby protecting cellular structures from damage and reducing oxidative damage in vital metabolic organs such as the pancreas, liver, brain, and muscles.
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