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The detection of fish freshness is crucial for ensuring food safety. This study addresses the limitations of traditional detection methods, which rely on laboratory equipment and complex procedures, by proposing a smartphone-based detection method, termed FreshFusionNet, that utilizes a pitaya peel pH intelligent indicator film in conjunction with multimodal deep learning. The pitaya peel indicator film, prepared using high-pressure homogenization technology, demonstrates a significant color change from dark red to yellow in response to the volatile alkaline substances released during fish spoilage. To construct a multimodal dataset, 3600 images of the indicator film were captured using a smartphone under various conditions (natural light and indoor light) and from multiple angles (0° to 120°), while simultaneously recording pH values, total volatile basic nitrogen (TVB-N), and total viable count (TVC) data. Based on the lightweight MobileNetV2 network, a Multi-scale Dilated Fusion Attention module (MDFA) was designed to enhance the robustness of color feature extraction. A Temporal Convolutional Network (TCN) was then used to model dynamic patterns in chemical indicators across spoilage stages, combined with a Context-Aware Gated Fusion (CAG-Fusion) mechanism to adaptively integrate image and chemical temporal features. Experimental results indicate that the overall classification accuracy of FreshFusionNet reaches 99.61%, with a single inference time of only 142 ± 40 milliseconds (tested on Xiaomi 14). This method eliminates the need for professional equipment and enables real-time, non-destructive detection of fish spoilage through smartphones, providing consumers and the food supply chain with a low-cost, portable quality-monitoring tool, thereby promoting the intelligent and universal development of food safety detection technology.
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http://dx.doi.org/10.3390/foods14101805 | DOI Listing |
Molecules
August 2025
Department of Food Science, Fu Jen Catholic University, New Taipei City 242062, Taiwan.
The effect of ultrasonic frequencies of 40 kHz/300 W (U-40) and 120 kHz/300 W (U-120) on the aqueous extraction of bioactive compounds from dried whole-fruit powders (DPs) of red-peel/white-flesh (WFP) and red-peel/red-flesh (RFP) pitayas was investigated, and shaking at 120 rpm (S-120) was used for a comparison. The effects of temperature and the solid-to-liquid ratio on the extraction efficiencies of the total phenolic content (TPC) and ferric-reducing antioxidant power (FRAP) of WFP and RFP were evaluated. The impact of extraction time on the aqueous extraction of specific compounds, namely, chlorogenic acid (CGA) and quercetin, from WFP and RFP was assessed with extraction modes of U-40, U-120, and S-120.
View Article and Find Full Text PDFJ Integr Plant Biol
July 2025
Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and Vegetables and Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (South China), Ministry of Agriculture and Rural Affairs, College of Horticulture, South China Agricultural University, Guangzhou, 510642,
Pitaya is an important perennial herbaceous fruit tree. The color of fruit determines pitaya nutritive (and attractive) value, which is considered as an important objective in breeding improvement. In this study, we reported the first telomere-to-telomere (T2T) gap-free genome of "Shuangse No.
View Article and Find Full Text PDFFoods
May 2025
College of Science, Sichuan Agricultural University, No.46, Xin Kang Road, Ya'an 625014, China.
The detection of fish freshness is crucial for ensuring food safety. This study addresses the limitations of traditional detection methods, which rely on laboratory equipment and complex procedures, by proposing a smartphone-based detection method, termed FreshFusionNet, that utilizes a pitaya peel pH intelligent indicator film in conjunction with multimodal deep learning. The pitaya peel indicator film, prepared using high-pressure homogenization technology, demonstrates a significant color change from dark red to yellow in response to the volatile alkaline substances released during fish spoilage.
View Article and Find Full Text PDFCurr Res Food Sci
April 2025
Food Science and Technology Program, Beijing Normal-Hong Kong Baptist University, Zhuhai, Guangdong, 519087, China.
The development of intelligent packaging materials can be alternative means to monitor food freshness and showcase real-time quality decline during storage. Colorimetric indicators are commonly employed as such examples due to their capability to exhibit chemically-responsive color changes triggered by the in-package headspace change during food deterioration. Different food-grade indicator dyes or pigments were investigated in literature, but one challenge was to incorporate the colorant into packaging materials with easy manipulation of indicator compositions.
View Article and Find Full Text PDFBMC Genomics
May 2025
Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection (Ministry of Education) & Guangxi Key Laboratory of Landscape Resources Conservation and Sustainable Utilization in Lijiang River Basin, Guangxi Normal University, Guilin, 541006, China.
Background: Pitaya is an important tropical fruit highly favoured by consumers owing to its good and juicy characteristics. It contains a large amount of betacyanin, which is a natural food-colouring agent, in the peel and pulp. However, few studies have focused on the pitaya chloroplast (cp) genomes.
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