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Article Abstract
Spent coffee grounds (SCGs) are a major global waste, yet rich in valuable bioactive compounds. Unlocking their potential requires advanced analytical techniques due to the complexity of their molecular composition. This study presents an innovative RPLC × SFC-HRMS method-an online two-dimensional liquid chromatography approach-for simultaneous analysis of sterol and diterpene derivatives in SCGs. Using a custom-built interface, both free and esterified lipid fractions were analyzed directly from intact, non-saponified and non-derivatized extracts. The method effectively resolved isomeric compounds and sterol oxidation products, highlighting its power for comprehensive lipid profiling and paving the way for SCG valorization in health-focused applications.
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| http://dx.doi.org/10.1016/j.foodchem.2025.144853 | DOI Listing |
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