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During the Arabidopsis reproductive process, the female whorl of the flower, known as the gynoecium, passes seven of the 20 floral stages during its development. In each of these seven stages, specific developmental events occur, ranging from gynoecium primordium establishment to complex tissue and organ differentiation. Studying gynoecium development is important for its role in fruit and seed formation. Currently, there are many Arabidopsis lines with fluorescent proteins that provide relevant information on gynoecium patterning. However, the fluorescence of some proteins is affected during the steps of histological techniques. Furthermore, the complexity of gynoecium development makes live imaging difficult in the early stages and medial tissues. To address these issues at hand, we describe a methodology that facilitates the analysis of the fluorescent signal during gynoecium development, using as an example the pMIR164c::VENUS line.
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http://dx.doi.org/10.1007/978-1-0716-4398-3_23 | DOI Listing |
Theor Appl Genet
September 2025
State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.
Hybrid breeding based on male sterility requires the removal of male parents, which is time- and labor-intensive; however, the use of female sterile male parent can solve this problem. In the offspring of distant hybridization between Brassica oleracea and Brassica napus, we obtained a mutant, 5GH12-279, which not only fails to generate gynoecium (thereby causing female sterility) but also has serrated leaves that could be used as a phenotypic marker in seedling screening. Genetic analysis revealed that this trait was controlled by a single dominant gene.
View Article and Find Full Text PDFJ Exp Bot
September 2025
Department of Biology, Kyungpook National University, Daegu 41566, Korea.
Above-ground lateral organs, such as leaves and flowers, should grow to an optimal size and develop particular structures, which are primarily laid out on the meristematic activities of their primordia. The GROWTH-REGULATING FACTOR‒GRF-INTERACTING FACTOR (GRF‒GIF) transcriptional complex and the AINTEGUMENTA (ANT) transcription factor play key roles in the establishment of the meristematic competence of lateral organ primordia with highly overlapping expression patterns, and thus functional relationships between them have long been speculated. In this study, we constructed the ant gif1 gif2 gif3 quadruple mutant, which produced much smaller and narrower leaves, sepals, and petals with far fewer cells compared with its parental lines.
View Article and Find Full Text PDFNaturwissenschaften
August 2025
Department of Biology, Shahid Bahonar University of Kerman, Kerman, Iran.
Plant galls are newly formed structures that develop due to the influence of gall-inducing organisms, providing them with shelter, protection, and nourishment. The most commonly studied galls are caused by insects. Plant-parasitic nematodes (PPNs) induce gall formation in various parts of plants, primarily targeting the roots.
View Article and Find Full Text PDFPlants (Basel)
August 2025
Bamboo Research Institute, Nanjing Forestry University, # 159 Longpan Road, Nanjing 210037, China.
Although the allocation of biomass among floral organs reflects critical trade-offs in plant reproductive strategies, the scaling relationships governing biomass allocations remain poorly resolved, particularly in flowers. Here, we report the fresh mass scaling allocation patterns among four floral organs (i.e.
View Article and Find Full Text PDFPlanta
July 2025
Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (Cinvestav), Unidad de Genómica Avanzada (UGA-Langebio), 36824, Irapuato, Mexico.
We identified new players of male and female reproductive development using tissue-specific gynoecium transcriptome data of Arabidopsis. Reproductive success in angiosperms depends on the correct development of the male and female organs. Pollen grains will land and germinate on the stigma, followed by the growth of pollen tubes that pass through the transmitting tract system of the gynoecium to reach the ovules.
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