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Zebrafish with unique biological traits can serve as an ideal model for studying olfactory mechanisms. This review analyzes their olfactory system, focusing on the regulation of receptor gene expression, mechanisms of odor recognition, and research methodologies including behavioral assays, molecular docking, and biotechnological approaches. Current limitations include predominantly qualitative data, insufficient cross-species comparisons, and unclear mechanisms of environmental modulation. Nevertheless, zebrafish models show significant potential in deciphering human olfaction and applications in neuroscience, biotechnology, healthcare, food safety, and environmental monitoring. Future research should establish cross-species olfactory databases, standardize behavioral assessments, and resolve technical bottlenecks to advance applications in precision medicine, food quality control, and pollutant detection.
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http://dx.doi.org/10.1016/j.foodchem.2025.144698 | DOI Listing |
HGG Adv
September 2025
Department of Medicine IV, Faculty of Medicine, Medical Center-University of Freiburg, University of Freiburg, Freiburg, Germany; Medizinische Genetik Mainz, Limbach Genetics, Mainz, Germany. Electronic address:
Cystic kidney disease and related ciliopathies are caused by pathogenic variants in genes that commonly result in ciliary dysfunction. For a substantial number of individuals affected by those cilia-related diseases, the causative gene still remains unknown. Using massively parallel sequencing, we here identified a pathogenic bi-allelic variant in the gene encoding PALS1-Associated Tight Junction Protein (PATJ; also known as Inactivation-No-Afterpotential D-Like, INADL) in an individual with ciliopathy.
View Article and Find Full Text PDFBrain Res Bull
September 2025
Department of Neuroscience of Disease, Brain Research Institute, Niigata University, Niigata, 951-8585, Japan. Electronic address:
Mutations in the UBE3A gene are responsible for neurodevelopmental disorders (NDDs), including Angelman syndrome (AS), which is characterized by developmental delays, impaired motor coordination, and cognitive disabilities. In recent years, UBE3A mutations have also been linked to autism spectrum disorders (ASD), due to their significant role in synaptic plasticity and cognitive function. Although substantial research has utilized mammalian models, the zebrafish (Danio rerio) provides unique opportunities to investigate gene functions owing to their transparent embryos, rapid development, and suitability for large-scale genetic and behavioral studies.
View Article and Find Full Text PDFBiochim Biophys Acta Rev Cancer
September 2025
Department of Biotechnology, Indian Institute of Technology Hyderabad (IITH), Kandi, Sangareddy 502284, Telangana, India. Electronic address:
Cancer metastasis remains the leading cause of cancer-related deaths, highlighting the urgent need for therapies targeting metastatic processes. Dysregulated calcium (Ca) signaling is increasingly linked to metastasis and offers a promising, underexplored therapeutic target. The zebrafish xenograft model has emerged as a powerful tool for studying cancer due to its optical transparency, genetic similarity to humans, and rapid development.
View Article and Find Full Text PDFAm J Hum Genet
September 2025
Stanley Manne Children's Research Institute, Ann & Robert H. Lurie Children's Hospital of Chicago, Chicago, IL 60611, USA; Department of Pediatrics and Department of Cell and Developmental Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL 60611, USA. Electronic address: erid
Fetal brain anomalies identified by prenatal ultrasound and/or magnetic resonance imaging represent a considerable healthcare burden with ∼1-2/1,000 live births. To identify the underlying etiology, trio prenatal exome sequencing or genome sequencing (ES/GS) has emerged as a comprehensive diagnostic paradigm with a reported diagnostic rate up to ∼32%. Here, we report five unrelated families with six affected individuals that presented neuroanatomical, craniofacial, and skeletal anomalies, all harboring rare, bi-allelic deleterious variants in SNAPIN, which encodes SNARE-associated protein.
View Article and Find Full Text PDFFood Chem
September 2025
College of Biomass Science and Engineering and Healthy Food Evaluation Research Center, Sichuan University, Chengdu 610065, China.
The growing demand for healthy Tartary buckwheat-based foods has sparked interest in fermentation as a processing technique to enhance food quality and bioactivity. This study investigated the impact of solid-state fermentation of black Tartary buckwheat (BTB) with Monascus purpureus and Eurotium cristatum PW-1 on its quality, biochemical properties, and hypolipidemic potential, using metabolomics, bioinformatics, network pharmacology, and invivo zebrafish models. Fermentation significantly increased total amino acids, γ-aminobutyric acid, and aromatic volatile compounds such as alcohols, esters, terpenes, and terpenoids, enhancing the flavor profile.
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