Molecular epidemiology of dengue in Southeast Asia (SEA): Protocol of systematic review and meta-analysis.

Introduction: Dengue fever is a major global public health challenge caused by the Arbovirus and transmitted by Aedes mosquitoes. The increasing incidence of dengue, particularly in the Southeast Asia (SEA) region, including Malaysia, highlights the urgent need for a comprehensive understanding of dengue molecular epidemiology. This study aims to systematically review various aspects of dengue molecular epidemiology to gain insights into the disease's dynamics, transmission and circulation. Providing evidence-based insights is crucial for the prevention and control of dengue.

Methods And Analysis: A systematic review and meta-analysis will be conducted following the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols guidelines. Eligible studies will include observational designs from the inception of time to 31 December 2024, in the SEA region. The review will encompass various molecular epidemiology domains as the exposures and assess the outcomes, such as confirmed dengue cases and severity. Descriptive and meta-analytical methods will determine prevalence, genetic changes and associations. Grading of Recommendations Assessment, Development, and Evaluation methodology will evaluate the quality of evidence, and reporting biases will be addressed. This review aims to bridge the gap in dengue molecular epidemiology in the SEA region by providing comprehensive insights crucial for effective dengue prevention and control.

Ethics And Dissemination: No primary data will be collected; thus, the ethical exemption was obtained from Medical Research Ethics Committee with reference number 23-03212-AE6 and ethics approval from the IMU University Joint Committee. The results will be disseminated through a peer-reviewed publication and conference presentation.

Prospero Registration Number: CRD42023480417.