Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Trimethylamine N-oxide (TMAO) serves as a crucial biomarker for early detection and prevention of cardiovascular and chronic kidney diseases. In this study, we design and implement a novel pixel array-based urine biosensor to explore the relationship between TMAO levels and glucose in urine and the urine albumin-creatinine ratio (UACR). The urine biosensor, incorporating a specialized readout circuit, measures TMAO across various UACR ranges, revealing a linear correlation with a slope of 8.5 mV per mg/g up to 1100 mg/g UACR. Although glucose levels also rise with UACR, significant discrepancies occur beyond 30 mg/g, indicating that glucose does not consistently correlate with UACR increases. The biosensor demonstrates a sensitivity of 41 ADC counts/μM (4.5 mV/μM), a 10-s response time, 98 % reproducibility, and a drift of 0.3 mV over extended periods. It requires only 5 μL of urine for a comprehensive analysis of TMAO and glucose. This approach significantly improves time efficiency, offering a faster and more convenient solution for monitoring the risk for chronic kidney disease, such as those with diabetes.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.aca.2025.343951 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Electrochemical Biosensor for Rapid Detection of Acute Rejection in Kidney Transplants.
Adv Healthc Mater
September 2025
Nanoengineered Systems Laboratory, UCL Mechanical Engineering, University College London, London, WC1E 7JE, UK.
Kidney transplant recipients face a high risk of acute rejection (AR), where the immune system attacks the transplanted organ. Current diagnostics rely on invasive biopsies with procedural risks, costs, and limited temporal resolution. While urinary chemokines CXCL9 and CXCL10 are promising non-invasive AR biomarkers, clinical adoption is limited by labor-intensive detection and lack of point-of-care (POC) solutions.
View Article and Find Full Text PDFEnzyme cascade-induced optical sensing of hyaluronidase using an ultraviolet-modified liquid crystal interface.
Mikrochim Acta
September 2025
Department of Chemistry, Gachon University, San 65, Bokjeong-Dong, Sujeong-Gu, Seongnam-City, Gyeonggi-Do, 461-701, South Korea.
H yaluronidase (HAase) is a clinically significant enzyme involved in tumor progression, extracellular matrix remodeling, and inflammation. Hence, HAase is an important biomarker for disease diagnostics. Here, a label-free, pH-responsive biosensor using ultraviolet (UV)-treated liquid crystals (LCs) for HAase detection is presented.
View Article and Find Full Text PDFNanozyme-integrated CuS@f-CB composite based electrochemical sensor for ultrasensitive detection of L-tryptophan in biological and food matrices.
Biosens Bioelectron
December 2025
International Ph.D. Program in Innovative Technology of Biomedical Engineering and Medical Devices, Ming Chi University of Technology, New Taipei City, 24301, Taiwan; Research Center for Intelligent Medical Devices, Ming Chi University of Technology, New Taipei City, 24301, Taiwan. Electronic addres
Nanozyme-based composites, a promising novel class of materials that mimic natural enzymes while offering greater stability, more catalytic activity, and cost-effectiveness, making them ideal for developing next-generation sensors. This work presents the synthesis and testing of a redox-active CuS@f-CB composite, synthesized by a combined hydrothermal-ultrasonication method, as a nanozyme-based electrocatalyst for label-free detection of L-tryptophan (L-TRP). This nanozyme-based composite combines the oxidase-like catalytic activity of copper sulfide with the high surface area, conductivity, and defect-rich structure of acid-treated carbon black.
View Article and Find Full Text PDFA SERS-Based Antibody-Aptamer Sandwich Assay for Extracellular Vesicle-Associated Tau Detection Using Gold Nanoparticles.
Adv Sens Res
August 2025
Department of Nanoengineering, Joint School of Nanoscience and Nanoengineering, North Carolina A&T State University, 2907 E Gate City Blvd, Greensboro, NC 27401.
Extracellular vesicles (EVs) have emerged as sources of promising, minimally invasive biomarkers for diagnosing and monitoring diseases like Alzheimer's. Using EVs as a source of biomarkers for neurological diseases is highly relevant because they can carry pathogenic proteins, such as tau and amyloid-β, across the blood-brain barrier and can be easily accessed and collected since they are available in almost all biofluids, including blood, urine, and saliva. Here, a bioanalytical antibody-aptamer sandwich assay detection using surface-enhanced Raman spectroscopy (SERS) is developed to quantify the expression of EV-associated tau.
View Article and Find Full Text PDFCutting-Edge Sensor Technologies for Exosome Detection: Reviewing Role of Antibodies and Aptamers.
Biosensors (Basel)
August 2025
Biomedical Engineering Programme, School of Medicine, The Chinese University of Hong Kong, Shenzhen 518172, China.
Exosomes are membranous vesicles that play a crucial role as intercellular messengers. Cells secrete exosomes, which can be found in a variety of bodily fluids such as amniotic fluid, semen, breast milk, tears, saliva, urine, blood, bile, ascites, and cerebrospinal fluid. Exosomes have a distinct bilipid protein structure and can be as small as 30-150 nm in diameter.
View Article and Find Full Text PDF