Cellulose and lignin nanoparticles from an Ayurvedic waste stream for essential oil-based active packaging to extend shelf life of strawberries.

Cellulose and lignin nanoparticles (NCP and LNP) were successfully extracted from Dashamoola spent material (DSM), a residue from an Ayurvedic decoction. NCP had a particle size of 493.6 nm and a zeta potential of -30.9 mV, indicating good colloidal stability. FTIR confirmed the removal of non-cellulosic components, while TGA demonstrated thermal stability, with major degradation between 260 °C and 350 °C. A semi-crystalline structure of nanocellulose was indicated via XRD analysis. Oil-in-water emulsions of tea tree oil (TTO) were prepared using NCP (C at 4 %), LNP (L at 4 %), and a combination blend (2 % each of C and L in CL_TTO), with 16 % TTO, all in w/v. Among these, CL_TTO emulsions had the smallest particle size and highest stability. PVOH-based films, prepared with a 4 % w/v mixture of CL_TTO emulsion, PVOH, and glycerol, demonstrated improved tensile strength, Young's modulus, water vapour barrier properties, and water repellence. These films blocked 95 % UV transmittance, providing appreciable protection to light-sensitive products. PVOH-CL_TTO films also exhibited strong antioxidant activity (85 % DPPH scavenging) and antimicrobial property against E. coli. These films extended the shelf life of strawberries by preserving lightness, firmness, and pH for 14 days under chilling (4 °C). These findings highlight the potential of NCP and LNP obtained from DSM for producing sustainable active packaging which would valorizing Ayurvedic waste stream.