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Cynomolgus monkeys (Macaca fascicularis) are vital in biomedical research, particularly for drug development and studying neurological diseases. However, accurately identifying individuals in group housing environments remains a significant challenge. This paper presents a near real-time facial recognition system tailored for cynomolgus monkeys, utilizing a fine-tuned Detectron2 model for face detection, followed by eigenface-based classification with Support Vector Machine (SVM) and radial basis function (RBF) kernel. The system achieved an accuracy of 97.65% in 10-fold cross-validation and identified individuals in under 1 minute under ideal conditions. This method eliminates the need for invasive identification techniques, potentially reducing stress and improving animal welfare, and has the potential to reduce the need for individualized housing or specialized enclosures. Additionally, as the system reduces the time and labor required for identifying monkeys, it might benefit research facilities with high turnover rates. This method could improve identification in non-human primate research while minimizing stress associated with traditional techniques.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978057 | PMC |
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0319897 | PLOS |
Sci Transl Med
September 2025
Vaccine Research Center (VRC), National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Human B cell immunity to the influenza hemagglutinin (HA) stem, a universal vaccine target, is often stereotyped and immunogenetically restricted, posing hurdles to study outside of humans. Here, we show that cynomolgus macaques vaccinated with an HA stem immunogen elicit humanlike public B cell lineages targeting two major conserved sites of vulnerability, the central stem and anchor epitopes. Central stem antibodies were predominantly derived from V1-138, the macaque homolog of human V1-69, a V gene preferentially used in human central stem broadly neutralizing antibodies (bnAbs).
View Article and Find Full Text PDFInvest Ophthalmol Vis Sci
September 2025
Center for Visual Science, University of Rochester, Rochester, NY, United States.
Purpose: Adaptive optics scanning light ophthalmoscopy (AOSLO) paired with intravitreal injection of a viral vector coding for the calcium indicator GCaMP has enabled visualization of neuronal activity in retinal ganglion cells (RGCs) at single cell resolution in the living eye. However, the inner limiting membrane (ILM) restricts viral transduction to the fovea in humans and non-human primates, hindering both therapeutic intervention and physiological study of the retina. To address this issue, we explored peeling the ILM before intravitreal injection to expand calcium imaging beyond the fovea in the living primate eye.
View Article and Find Full Text PDFZool Res
September 2025
College of Veterinary Medicine, South China Agricultural University, Guangzhou, Guangdong 510642, China. E-mail:
Zona pellucida glycoprotein-1 (ZP1) is essential for maintaining oocyte structural integrity and facilitating fertilization. Mutations in are strongly associated with primary infertility disorders such as fertilization failure and empty follicle syndrome; however, the absence of accurate experimental models has hindered mechanistic understanding and obscured the etiological basis of -related infertility. In this study, CRISPR/Cas9-mediated genome editing was employed to generate two -edited cynomolgus macaques ( ), designated #ZP1-1 (male) and #ZP1-2 (female).
View Article and Find Full Text PDFMol Cancer Ther
September 2025
Novelty Nobility, Seongnam-si, Korea (South), Republic of.
Overexpression and gain-of-function mutations of c-Kit have been implicated in cancers including gastrointestinal stromal tumors (GIST), small cell lung cancer (SCLC), acute myeloid leukemia, and systematic mastocytosis. In clinics, small molecule c-Kit inhibitors often result in secondary c-Kit mutations or are ineffective despite c-Kit overexpression. We developed NN3201, a novel c-Kit targeting antibody-drug conjugate (ADC), via rational design to evaluate its anticancer activity in c-Kit-positive tumors, and preclinical pharmacologic profiles.
View Article and Find Full Text PDFGenome Biol
September 2025
Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, Milwaukee, WI, 53226, USA.
Background: A growing body of evidence from primate embryos as well as in vitro systems supports the notion that amnion and primordial germ cell (PGC) lineage progressing cells share a common precursor.
Results: To gain comprehensive transcriptomic insights into this critical but poorly understood precursor and its progeny, we examine the evolving transcriptome of a developing human pluripotent stem cell-derived model of amnion and PGC formation at the single cell level. This analysis reveals several continuous amniotic fate progressing states with state-specific markers.