Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
This study explores the molecular-level cryoprotective effect of cod collagen peptide-1 (CCP-1) on surimi during freeze-thaw cycles, examining its interaction with ice crystals and its role in maintaining the structural integrity of surimi. Results indicated that CCP-1 exhibited the most effective cryoprotection on catalase, preserving 83.4 % of its residual enzyme activity, and prevented myofibrillar protein (MP) from freeze denaturation by sustaining the activity of Ca-ATPase and maintaining structural integrity. The antifreeze effect of CCP-1 (1.0 % and 3.0 %, /w) is comparable to that of commercial antifreeze containing 0.5 % compound phosphate (/w). Moreover, CCP-1's ability to interact with ice crystals is closely tied to its primary structure, where hydrophilic and hydrophobic amino acids work in tandem. Specifically, alkaline and acidic amino acids are capable of forming stronger hydrogen bonds, thereby enhancing their interaction with ice crystals. This work offers a theoretical basis for analyzing the binding behavior of antifreeze peptides with varying amino acid compositions.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11930166 | PMC |
| http://dx.doi.org/10.1016/j.fochx.2025.102334 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Devitrification and melting in vapor deposited ice.
J Chem Phys
September 2025
Dipartimento di Fisica, Università degli Studi di Roma La Sapienza, Piazzale Aldo Moro 5, Rome 00185, Italy.
The equilibration dynamics of ultrastable glasses subjected to heating protocols has attracted recent experimental and theoretical interest. With simulations of the mW water model, we investigate the devitrification and "melting" dynamics of both conventional quenched (QG) and vapor deposited (DG) amorphous ices under controlled heating ramps. By developing an algorithm to reconstruct hydrogen-bond networks, we show that bond ring statistics correlate with the structural stability of the glasses and allow tracking crystalline and liquid clusters during devitrification and melting.
View Article and Find Full Text PDFEthanol-mediated freeze-drying enables robust bacterial cellulose aerogels for enhanced drug loading and hemostasis dressing.
Carbohydr Polym
November 2025
Institute of Nano and Biopolymeric Materials, School of Materials Science and Engineering, Tongji University, Shanghai 201804, China. Electronic address:
This work reports an ethanol-mediated freeze-drying (EMFD) strategy that enables the scalable production of high-performance bacterial cellulose aerogels (BCAs), effectively addressing key limitations of conventional methods such as supercritical drying and standard freeze-drying, including fragility, low mechanical strength, and high cost. Specifically, by replacing water in bacterial cellulose hydrogels (BCHs) with ethanol-water solution (EWs) prior to freeze-drying, the process limits ice crystal formation and reduces capillary forces and adhesion, thereby preserving structural integrity and enhancing mechanical properties. The effects of EWs concentration on BCA morphology, volume shrinkage, mechanical strength, and pore structure were systematically investigated.
View Article and Find Full Text PDFImproving sludge dewaterability through freeze-thaw pretreatment: Ice crystal morphology controls consolidation and rheological enhancement.
Environ Res
September 2025
Vietnam National University Ho Chi Minh City, Ho Chi Minh City, 700000, Viet Nam. Electronic address:
Municipal sludge, characterized by its high-water-content and viscous texture, poses significant environmental challenges due to inefficient dewatering and poor flowability. The freeze-thaw (F/T) method is an effective and environmentally friendly pretreatment approach. It is crucial to apply rheological analysis to examine the influence of refrigeration temperature on dewatering effects and to investigate the underlying mechanisms.
View Article and Find Full Text PDFImpact of nucleation temperature and hydroxyethyl starch on ice crystal growth: Implications for cell viability during extreme temperature fluctuations.
J Therm Biol
August 2025
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB, Canada; Innovation and Portfolio Management, Canadian Blood Services, Edmonton, AB, Canada. Electronic address:
Extreme temperature fluctuations during routine handling and shipping of cryopreserved cell products significantly compromise product quality in ways that extend beyond the duration and peak temperature of the fluctuation. The type of cryoprotectant used and the initial ice nucleation temperature influence ice crystal growth during rewarming events, in turn impacting cell survival. Using a cryomicroscope together with temperature profiles recorded in cord-blood units, ice crystal growth was tracked through five transient-warming events (TWEs) that peaked at -30 °C, -20 °C, or -10 °C.
View Article and Find Full Text PDFMechanisms underlying the freezing and melting behavior of water confined in silica nanopores as a function of pore size and pore filling.
Phys Chem Chem Phys
September 2025
School of Civil and Environmental Engineering, Cornell University, Ithaca, NY, USA.
Uncovering the mechanisms of freezing and melting behavior in nanoconfined fluids can unlock fundamental insights into the fate and transport of fluids in soils present in cold climates. From a scientific perspective, the structural and thermodynamic behavior of confined and interfacial water has sparked significant discussions, particularly regarding the characteristics of phase transitions and spatial heterogeneity as a function of temperature and pressure. Observations frequently report interfacial unfrozen liquid layers on hydrophilic surfaces, distorted ice crystals and suppressed freezing and melting points in confined water compared to bulk water.
View Article and Find Full Text PDF