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Phytogenic feed additives are gaining attention as potential alternatives to enhance broiler growth performance and feed efficiency. The experiment investigated the effects of supplementing broiler diets over 35 days with two levels of Fibrafid (0.15 % and 0.25 %) under two dietary regimes: normal amino acid density and metabolizable energy (ME) levels (referred to as positive control diet plus Fibrafid: PC1+0.15 % or PC1+0.25 % Fibrafid) and diets with a 5 % reduction in amino acid density and a 1.5 % reduction in ME (referred to as negative control diet plus Fibrafid: NC1+0.15 % or NC1+0.25 % Fibrafid) on performance parameters and carcass attributes of Ross 308 broilers. These were compared to standard positive control diets (PC1 and PC2, without or with TURBO Grow) and negative control diets (NC1 and NC2, without or with TURBO Grow). Performance indicators were estimated during 0-9, 11-23, 24-35, and 0-35. On day 35, the carcass characteristics and jejunal histopathology were evaluated. Results demonstrated significant improvements in body weight gain and feed conversion ratio (FCR) with Fibrafid inclusion, particularly at 0.25 % over trail. Fibrafid supplementation compensated for reduced dietary energy and protein, leading to improved growth performance, and gizzard and Bursa weights compared to negative control diets. Compared to NC1, the PC1+0.25 % Fibrafid show a significant improvement in jejunal villus morphology, leading to an increase in villus width, height, surface area, and goblet cell density by 5.3 %, 4.8 %, 12.5 %, and 56.7 % respectively. In conclusion, Fibrafid supplementation positively impacts growth performance and intestinal structure, potentially improving nutrient absorption, and jejunal integrity in broilers, proving Fibrafid's promise as a viable option in modern poultry nutrition.
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http://dx.doi.org/10.1016/j.psj.2025.105014 | DOI Listing |
Genetics
September 2025
Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA.
Protein translation regulation is critical for cellular responses and development, yet how elongation stage disruptions shape these processes remains incompletely understood. Here, we identify a single amino acid substitution (P55Q) in the ribosomal protein RPL-36A of Caenorhabditis elegans that confers complete resistance to the elongation inhibitor cycloheximide (CHX). Heterozygous animals carrying both wild-type RPL-36A and RPL-36A(P55Q) develop normally but show intermediate CHX resistance, indicating a partial dominant effect.
View Article and Find Full Text PDFSci Transl Med
September 2025
Department of Medicine, Columbia University Irving Medical Center, New York, NY 10032, USA.
Hepatocyte apoptosis is a key feature of metabolic dysfunction-associated steatohepatitis (MASH), but the fate of apoptotic hepatocytes in MASH is poorly understood. Here, we explore the hypotheses that clearance of dead hepatocytes by liver macrophages (efferocytosis) is impaired in MASH because of low expression of the efferocytosis receptor T cell immunoglobulin and mucin domain containing 4 (TIM4; gene ) by MASH liver macrophages, which then drives liver fibrosis in MASH. We show that apoptotic hepatocytes accumulate in human and experimental MASH, using mice fed the fructose-palmitate-cholesterol (FPC) diet or the high-fat, choline-deficient amino acid-defined (HF-CDAA) diet.
View Article and Find Full Text PDFSci Transl Med
September 2025
Vaccine Research Center (VRC), National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Human B cell immunity to the influenza hemagglutinin (HA) stem, a universal vaccine target, is often stereotyped and immunogenetically restricted, posing hurdles to study outside of humans. Here, we show that cynomolgus macaques vaccinated with an HA stem immunogen elicit humanlike public B cell lineages targeting two major conserved sites of vulnerability, the central stem and anchor epitopes. Central stem antibodies were predominantly derived from V1-138, the macaque homolog of human V1-69, a V gene preferentially used in human central stem broadly neutralizing antibodies (bnAbs).
View Article and Find Full Text PDFPLoS One
September 2025
Department of Computer Science, Emory University, Atlanta, Georgia, United States of America.
Background: Erythema, an early visual indicator of tissue damage preceding pressure injuries (PrIs), presents as redness in light skin tones but is harder to detect in dark skin tones. While thermography shows promise for early PrI detection, validation across different skin tones remains limited. Furthermore, most protocols and models have been developed under highly controlled conditions.
View Article and Find Full Text PDFAm J Physiol Cell Physiol
September 2025
Division of Medical Sciences, NOSM University, Ontario, Canada.
Cancer induced skeletal muscle wasting (cachexia) is responsible for over 20% of cancer related deaths, yet much about the pathophysiology of the condition remains unknown. Importantly, cancer cachexia does not seem wholly responsive to traditional anabolic stimuli such as nutritional interventions. It is possible that tumours directly or indirectly target skeletal muscle for their dynamic and abundant pool of amino acids that can be reliably used by tumours to supplement energy production and biomass synthesis.
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