Multilocus phylogeny contributes to fungal taxonomy with three novel species of (Bolbitiaceae) from southern Punjab, Pakistan.

Mycologia

Key Laboratory of Integrated Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang 830091, China.

Published: April 2025


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Article Abstract

Advancements in fungal taxonomy have been significantly enhanced by multilocus phylogenetic analyses, which improve the precision of species identification. This study also employs such methods to investigate the genus , resulting in the description of three novel species, viz. and belonging to the section and of section , from southern Punjab, Pakistan. Field collections yielded specimens that were examined morphologically and genetically, using a multilocus phylogenetic analysis utilizing sequences from the ITS, 28S, and regions. A combined phylogenetic tree was constructed through maximum likelihood and Bayesian inference methods, revealing three novel taxa occupying their respective distinct branches. Further, morphological analysis complemented the genetic data, with each species displaying unique characteristics. This study not only adds to the fungal biodiversity of Pakistan but also provides a robust framework for future taxonomic and ecological research in the region. In addition, we provide a key to the known species of in Pakistan.

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http://dx.doi.org/10.1080/00275514.2025.2463279DOI Listing

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