Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Bioinsecticides based on the bacterium (Bt) are widely used as safe alternatives to chemical insecticides. The insecticidal activity of Bt is occasioned by a protein toxin contained in parasporal crystals (Cry proteins) that are synthesized and laid down alongside the endospore during sporulation. The specificity of toxin action is associated with the subspecies of Bt and the individual Cry toxins they produce. Although a number of commercial Bt formulations are available to control moths, mosquitoes and beetles, there are none that control the red imported fire ant (RIFA) . The present report is the first to describe the insecticidal activity of the Cry3A protein toxin, produced by subsp. (Btt), against the RIFA as well as some of its key biochemical properties. Currently available commercial formulations of Btt are designed to control beetles such as the Colorado potato beetle, not ants. The Cry3A toxin (MW ~66 kDa) is embedded in a larger polypeptide (protoxin, MW ~73 kDa) and is released from the toxin enzymatically. Once activated, it can be administered to the RIFA as a soluble protein that most likely binds to an attendant receptor in the epithelial cells that line the wall of the larval ventriculus, killing the insect. Properly customized, the Cry3A toxin is a potential candidate for fire ant control.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11858235 | PMC |
| http://dx.doi.org/10.3390/microorganisms13020371 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Biochemical Features of the Cry3A Toxin of subsp. and Its Toxicity to the Red Imported Fire Ant .
Microorganisms
February 2025
Department of Biological Sciences, The University of Texas at Dallas, Richardson, TX 75080-3021, USA.
Bioinsecticides based on the bacterium (Bt) are widely used as safe alternatives to chemical insecticides. The insecticidal activity of Bt is occasioned by a protein toxin contained in parasporal crystals (Cry proteins) that are synthesized and laid down alongside the endospore during sporulation. The specificity of toxin action is associated with the subspecies of Bt and the individual Cry toxins they produce.
View Article and Find Full Text PDFIPD072Aa from Pseudomonas chlororaphis Targets Midgut Epithelial Cells in Killing Western Corn Rootworm ().
Appl Environ Microbiol
March 2023
Corteva Agriscience, Johnston, Iowa, USA.
IPD072Aa from Pseudomonas chlororaphis is a new insecticidal protein that has been shown to have high activity against western corn rootworm (WCR). IPD072 has no sequence signatures or predicted structural motifs with any known protein revealing little insight into its mode of action using bioinformatic tools. As many bacterially derived insecticidal proteins are known to act through mechanisms that lead to death of midgut cells, we evaluated whether IPD072Aa also acts by targeting the cells of WCR midgut.
View Article and Find Full Text PDFEffect of Cry Toxins on (Coleoptera: Cerambycidae) Larvae.
Insects
December 2021
Instituto Universitario de Biotecnología y Biomedicina (BIOTECMED), Departamento de Genética, Universitat de Valencia, 46100 Burjassot, Spain.
The beetle is a destructive pest in vineyards () in the main wine-producing areas of the Iberian Peninsula. larvae bore into the grapevine wood-making galleries, thus damaging the plant both directly and indirectly; the latter through the proliferation of wood fungi, which can invade the inside of the plant, decreasing the quality and quantity of its production. The susceptibility of larvae to five coleopteran toxic Cry proteins (Cry1B, Cry1I, Cry3A, Cry7A, and Cry23/37) was evaluated under laboratory conditions in order to deepen the knowledge of the effect of these proteins on this insect throughout its biological development.
View Article and Find Full Text PDFUnveiling gene expression regulation of the Bacillus thuringiensis Cry3Aa toxin receptor ADAM10 by the potato dietary miR171c in Colorado potato beetle.
Pest Manag Sci
September 2022
Department of Genetics, University of Valencia, Burjassot, Spain.
Background: The Colorado potato beetle (CPB) is a worldwide devastating pest of potato plants and other Solanaceae characterized by its remarkable ability to evolve resistance to insecticides. Bacillus thuringiensis (Bt) Cry3Aa toxin represents an environmentally safe alternative for CPB control but larvae susceptibility to this toxin has been reported to vary depending on the host plant on which larvae feed. To gain more insight into how nutrition mediates Bt tolerance through effects on gene expression, here we explored the post-transcriptional regulation by microRNAs (miRNAs) of the CPB-ADAM10 gene encoding the Cry3Aa toxin functional receptor ADAM10.
View Article and Find Full Text PDFSpores and Cry3A Toxins Act Synergistically to Expedite Colorado Potato Beetle Mortality.
Toxins (Basel)
October 2021
Department of Biosciences, Faculty of Science and Engineering, Swansea University, Swansea SA2 8PP, Wales, UK.
The insect integument (exoskeleton) is an effective physiochemical barrier that limits disease-causing agents to a few portals of entry, including the gastrointestinal and reproductive tracts. The bacterial biopesticide (Bt) enters the insect host via the mouth and must thwart gut-based defences to make its way into the body cavity (haemocoel) and establish infection. We sought to uncover the main antibacterial defences of the midgut and the pathophysiological features of Bt in a notable insect pest, the Colorado potato beetle (CPB).
View Article and Find Full Text PDF