Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
AMP-activated protein kinase (AMPK) has evolved to detect a critical increase in cellular AMP/ATP and ADP/ATP concentration ratios as a signal for limiting energy supply. Such energy stress then leads to AMPK activation and downstream events that maintain cellular energy homeostasis. AMPK activation by AMP, ADP, or pharmacological activators involves a conformational switch within the AMPK heterotrimeric complex. We have engineered an AMPK-based sensor, AMPfret, which translates the activating conformational switch into a fluorescence signal, based on increased fluorescence resonance energy transfer (FRET) between donor and acceptor fluorophores. Here we describe how this sensor can be used to analyze direct AMPK activation by small molecules in vitro using a fluorimeter, or to estimate changes in the energy state of cells using standard fluorescence or confocal microscopy.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-4284-9_2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
FOXO3 Protects Lens Epithelial Cells From UVB-Induced Oxidative Stress via the AMPK/FOXO3 Signaling Pathway.
FASEB J
September 2025
Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China.
Age-related cataract (ARC) represents a major global cause of visual impairment, with ultraviolet B (UVB) radiation recognized as a primary contributor to oxidative damage in the lens. FOXO3, a key regulator of aging, apoptosis, and oxidative stress-induced cell death, was investigated for its role and regulatory mechanisms in UVB-induced oxidative stress using human lens epithelial cells (HLECs). A progressive decrease in FOXO3 protein expression was observed in the lens capsules across various stages of cataract progression, as well as in UVB-exposed animal models and UVB-treated HLECs.
View Article and Find Full Text PDF[SGLT2i: Exploring Multiple Pathways in Cardiorenal Protection. Insights Into Nutrient Deprivation].
G Ital Nefrol
August 2025
UO Nefrologia e Dialisi, Ospedale di Cassino, Italia.
SGLT-2 inhibitors are a relatively new class of antidiabetic drugs. They activate a transcriptional response similar to calorie restriction characterized by the up-regulation of sensors involved in nutrient deprivation, such as SIRT1 and AMPK, and the down-regulation of mTOR, a molecule involved in nutritional excess signaling. The purpose of this review is to illustrate the main pathways of nutrient deprivation: a complex mechanistic framework partly responsible for the cardio-renal benefits that makes these drugs unique.
View Article and Find Full Text PDFAlleviating effects of polyphenol extract from rapeseed meal on type 2 diabetes in mice via modulation of gut microbiota and AMPK/mTOR signaling pathways.
Food Res Int
November 2025
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Oil Crops and Lipids Process Technology National & Local Joint Engineering Laboratory, Key Laboratory of Oilseeds Processing, Ministry of Agriculture and Rural Affairs, Hubei Key Laboratory of Lipid Chemistry and Nutrition
Type 2 diabetes mellitus (T2DM) is a a complex metabolic disorder that poses a serious threat to human health. Although polyphenol extract from rapeseed meal (RMP) has demonstrated inhibitory activity against α-glucosidase, the alleviating effects on T2DM and the underlying molecular mechanisms remain largely unexplored in T2DM. In this study, the antidiabetic effects of RMP were investigated using a T2DM mouse model induced by a high-fat diet (HFD) combined with streptozotocin (STZ) administration.
View Article and Find Full Text PDFCollagen peptides from Skipjack tuna (Katsuwonus pelamis) bones: Composition, characterization, and mechanism of high-fat diet-induced NAFLD amelioration.
Food Res Int
November 2025
National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China. Electronic address: chichang
This study aimed to analyze the amino acid composition and characterize the sequences of collagen peptides from Skipjack tuna bones (TBCPs) by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and further investigate the function and mechanism of action of TBCPs in nonalcoholic fatty liver disease (NAFLD). The results showed that TBCPs contain 16 types of amino acids, among which glycine is the most abundant, and hydrophobic amino acids account for 40.75 %.
View Article and Find Full Text PDFSerum-free media for cultured meat: insights into protein hydrolysates as proliferation enhancers.
Food Res Int
November 2025
College of Food Science, Southwest University, Chongqing 400715, China. Electronic address:
Cultured meat represents an innovative alternative to conventional livestock-derived meat, yet faces challenges in establishing efficient, safe, and sustainable culture systems. Although traditional supplements such as fetal bovine serum and bovine eye fluid provide essential growth factors for cell proliferation, their high cost, ethical concerns, and biosafety risks significantly hinder large-scale industrialization. In recent years, protein hydrolysates have emerged as promising components in serum-free media.
View Article and Find Full Text PDF