Category Ranking
98%
Total Visits
921
Avg Visit Duration
2 minutes
Citations
20
Article Abstract
Proximity ligation assays (PLAs) use specific antibodies to detect endogenous protein-protein interactions. PLAs are a highly useful biochemical technique that allow two proteins within proximity to be visualized with fluorescent probes amplified by PCR. While this technique has gained prominence, the use of a PLA in mouse skeletal muscle (SkM) is novel. In this article, we discuss how the PLA method can be used in SkM to study the protein-protein interactions within mitochondria-endoplasmic reticulum contact sites (MERCs). © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol: Proximity ligation assay for skeletal muscle tissue and myoblast for MERC proteins.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11795341 | PMC |
| http://dx.doi.org/10.1002/cpz1.70043 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genome-wide mapping of RNA-protein associations through sequencing.
Nat Biotechnol
September 2025
Institute of Engineering in Medicine, University of California, San Diego, La Jolla, CA, USA.
RNA-protein interactions critically regulate gene expression and cellular processes, yet their comprehensive mapping remains challenging due to their structural diversity. We introduce PRIM-seq (protein-RNA interaction mapping by sequencing), a method for concurrent de novo identification of RNA-binding proteins and their associated RNAs. PRIM-seq generates unique chimeric DNA sequences by proximity ligation of RNAs with protein-linked DNA barcodes, which are subsequently decoded through sequencing.
View Article and Find Full Text PDFReceptor transporter protein 4 (RTP4)-mediated repression of hepatitis C virus replication in mouse cells.
PLoS Pathog
September 2025
Department of Molecular Biology, Princeton University, Princeton, New Jersey, United States of America.
Hepatitis C virus (HCV) exhibits a narrow species tropism, causing robust infections only in humans and experimentally inoculated chimpanzees. While many host factors and restriction factors are known, many more likely remain unknown, which has limited the development of mouse or other small animal models for HCV. One putative restriction factor, the black flying fox orthologue of receptor transporter protein 4 (RTP4), was previously shown to potently inhibit viral genome replication of several ER-replicating RNA viruses.
View Article and Find Full Text PDFProviral functions of HMGB1 in HAdV-C5 replication compartments.
Microbiol Spectr
September 2025
Department of Viral Transformation, Leibniz Institute of Virology (LIV), Martinistraße, Hamburg, Germany.
Unlabelled: Human adenoviruses (HAdVs) induce significant reorganization of the nuclear environment, leading to the formation of virus-induced subnuclear structures known as replication compartments (RCs). Within these RCs, viral genome replication, gene expression, and modulation of cellular antiviral responses are tightly coordinated, making them valuable models for studying virus-host interactions. In a recent study, we analyzed the protein composition of HAdV type 5 (HAdV-C5) RCs isolated from infected primary cells at different time points during infection using quantitative proteomics.
View Article and Find Full Text PDFBioID2-Based Tau Interactome Reveals Novel and Known Protein Interactions Associated with Multiple Cellular Pathways.
J Proteome Res
September 2025
Department of Translational Neuroscience, College of Human Medicine, Michigan State University, Grand Rapids, Michigan 49503, United States.
Pathological inclusions composed of tau are hallmarks of neurodegenerative diseases termed tauopathies, the most common of which is Alzheimer's disease. Accumulating evidence suggests that tau is involved in a multitude of physiological functions that are regulated, in part, by direct and/or transient protein interactions. Deciphering the tau interactome is critical for understanding the physiological and pathological roles of tau.
View Article and Find Full Text PDFSurgical complication of SMA and celiac artery ligation during radical nephrectomy for large renal tumor: A fatal outcome.
Radiol Case Rep
November 2025
Radiology Department, Shaukat Khanum Memorial Cancer Hospital and Research Centre Lahore, Punjab, Pakistan.
Iatrogenic ligation of extrarenal vessels during left nephrectomy is a rarely reported yet life-threatening complication, typically occurring due to anatomical vascular variation, distorted anatomy or large lymphadenectomy. Preoperative assessment of anatomy through radiological imaging and careful identification of each vessel intraoperatively is important to prevent this complication. Such complications often result in fatal tissue ischemia and multi-organ failure if not diagnosed and managed in time.
View Article and Find Full Text PDF