FAST-PB: An automated plant bioengineering system for scalable genome editing and phenotyping.

Plant Cell

Assistant Features Editor, The Plant Cell, American Society of Plant Biologists.

Published: February 2025


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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11827612PMC
http://dx.doi.org/10.1093/plcell/koaf020DOI Listing

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Plant bioengineering is a time-consuming and labor-intensive process with no guarantee of achieving desired traits. Here, we present a fast, automated, scalable, high-throughput pipeline for plant bioengineering (FAST-PB) in maize (Zea mays) and Nicotiana benthamiana. FAST-PB enables genome editing and product characterization by integrating automated biofoundry engineering of callus and protoplast cells with single-cell matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS).

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