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Hydrogen internal combustion engines offer a near-term decarbonisation pathway for hard to electrify sectors such as non-road mobile machinery (NRMM). However, few hydrogen-specific engines have ever been developed with the twin-goals of maximising low carbon energy efficiency and delivering air quality co-benefits. We present analyses of dynamometer-derived nitrogen oxides (NO) tailpipe emissions from four variants of a ∼55 kW four-cylinder port fuelled injection spark ignition hydrogen internal combustion engine (H2ICE) suitable for a range of uses within the NRMM industry. Engine out (pre-aftertreatment) emissions are also reported for one of the H2ICE variants. The emissions were compared over the Non-Road Transient Cycle (NRTC) with an equivalent contemporary Stage V emissions compliant 55 kW diesel engine. All four H2ICE variants were configured to operate under lean burn conditions generating substantially lower NO exhaust emissions over the NRTC when compared to the diesel engine. Lowest NO emissions were observed for a spark ignition H2ICE with selective catalytic reduction and particulate filter (SCRF) aftertreatment. Tailpipe NO emissions over the full NRTC for this configuration were 1.90 mg kWh, a greater than 99% reduction compared to diesel (3340 mg kWh) with lower average NO emissions observed for the H2ICEs over all power, torque, and speed settings. The frequency and magnitude of transient (<20 ms) increases in NO were also compared between diesel and H2ICE. A H2ICE using a hydrogen slip catalyst, but without SCRF aftertreatment, also emitted significantly lower tailpipe NO than the diesel equivalent (63.7 mg kWh), a factor of greater than 50 times improvement over the NRTC. This creates a systems level dilemma: whether the additional small absolute reductions in NO achieved using SCRF would have a net benefit that outweighed the broader financial and environmental costs of the SCR and exhaust fluid manufacture, distribution and possible small in-service ammonia slip from exhaust. Irrespective of aftertreatment system, the adoption of low NO emitting H2ICE in NRMM, and particularly construction equipment, would appear to offer much greater near-term air quality benefits for cities when compared to switching to other low carbon alternatives such as biodiesel or hydrotreated vegetable oil.
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http://dx.doi.org/10.1039/d4em00448e | DOI Listing |
J Cell Biol
October 2025
Autophagy, Inflammation and Metabolism Center of Biochemical Research Excellence, University of New Mexico Health Sciences Center, Albuquerque, NM, USA.
The mechanisms governing mammalian proton pump V-ATPase function are of fundamental and medical interest. The assembly and disassembly of cytoplasmic V1 domain with the membrane-embedded V0 domain of V-ATPase is a key aspect of V-ATPase localization and function. Here, we show that the mammalian protein ATG16L1, primarily appreciated for its role in canonical autophagy and in noncanonical membrane atg8ylation processes, controls V-ATPase.
View Article and Find Full Text PDFNMR Biomed
October 2025
High-Field MR Center, Department of Biomedical Imaging and Image-Guided Therapy, Medical University of Vienna, Vienna, Austria.
The human kidneys play a pivotal role in regulating blood pressure, water, and salt homeostasis, but assessment of renal function typically requires invasive methods. Deuterium metabolic imaging (DMI) is a novel, noninvasive technique for mapping tissue-specific uptake and metabolism of deuterium-labeled tracers. This study evaluates the feasibility of renal DMI at 7-Tesla (7T) to track deuterium-labeled tracers with high spatial and temporal resolution, aiming to establish a foundation for potential clinical applications in the noninvasive investigation of renal physiology and pathophysiology.
View Article and Find Full Text PDFAm J Physiol Regul Integr Comp Physiol
September 2025
Department of Health, Nutrition, and Food Sciences, Florida State University, Tallahassee, FL, USA.
Cystathionine γ-lyase (CSE) produces hydrogen sulfide (HS), a vasodilator critical for vascular function. While its systemic effects are well-documented, its role in erectile physiology remains unclear. This study investigated the impact of CSE deletion on vascular and erectile tissue reactivity.
View Article and Find Full Text PDFJ Biomol Struct Dyn
September 2025
Enzyme and Microbial Technology Research Center, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
A thermostable paraoxonase (S3wahi-PON) from sp. strain S3wahi was recently characterised and shown to possess stability across a broad temperature range. This study expands upon the initial biochemical characterisation of S3wahi-PON by investigating the structural determinants and conformational adaptability that contribute to its thermostability, using an integrated approach that combines biophysical techniques and molecular dynamics (MD) simulations across a temperature range of 10 °C to 90 °C.
View Article and Find Full Text PDFThe T138R mutation in Aquaporin 0 (AQP0), a key membrane protein in the ocular lens, causes autosomal dominant congenital cataracts. Whilst previous studies have demonstrated that this mutation disrupts water permeability and leads to protein mislocalisation, the specific structural mechanisms underlying these functional defects remain unclear. This study employed in silico approaches to characterise how the T138R substitution affects AQP0's molecular structure and stability.
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