Food Safety Concerns in Pakistan: Monitoring of Antimicrobial-Resistant Bacteria and Residue Contamination in Commercially Available Fish and Poultry Meat Samples.

Food safety is a critical issue in the 21st century due to antibiotic-resistant bacteria causing harmful foodborne diseases. This comprehensive study meticulously examined the presence of bacterial isolates, quinolone residue, and antimicrobial resistance genes in samples of broiler and fish meat. Forty samples were collected from various locations in the Faisalabad metropolis. The samples underwent culture on various types of agar, and the Vitek 2 compact system was used to confirm the isolates along with determining the minimum inhibitory concentration of antibiotics. Additionally, polymerase chain reaction was used to identify antimicrobial-resistant genes, and an enzyme-linked immunosorbent assay (ELISA) kit was employed to detect quinolone residue in each sample. Out of 40 fish samples, 66 polymicrobials were identified with being the most prevalent (26.6%), followed by (16.6%) and (15%). Furthermore, 70 polymicrobial were identified in the broiler samples, with 41.6% and 15% . In fish isolates, 14 (21%) gene, 18 (27.2%) , and 11 (16.6%) , and in broiler samples, 19 (27.1%) gene, 19 (27.1%) and 22 (31.4%) , and 5 (7.1%) were found. Eighteen (45%) broiler and 7 (17.5%) fish samples with more than 100 µg per kg (ppb) for quinolone residues. The study concluded that the increased prevalence of multidrug-resistant bacteria and quinolone residue poses a significant threat to consumer health.