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Tannase, as a type of tannin-degrading enzyme, can catalyze the hydrolysis of ester and depside bonds in gallotannins, thereby releasing gallic acid and glucose. Based on this reaction mechanism, Tannase can effectively improve the problems of bitter taste, weak aroma, and tea cheese in tea infusion, and is therefore widely used in the tea industry. However, due to high production costs, difficulties in purification and recovery, and insufficient understanding of Tannase properties, the large-scale application of Tannase is severely limited. Therefore, the sources of Tannase and the effects of fermentation temperature, pH, stirring speed, time, carbon, and nitrogen sources on the preparation of Tannase are described in this study. The advantages and disadvantages of various methods for measuring Tannase activity and their enzymatic characterization are summarized, and the concentration and purification methods of Tannase are emphasized. Finally, the application of Tannase to reduce the formation of tea precipitate, enhance antioxidant capacity, increase the extraction rate of active ingredients, and improve the flavor of the tea infusion is described. This study systematically reviews the production, characterization, purification, and application of Tannase to provide a reference for further research and application of Tannase.
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http://dx.doi.org/10.3390/foods14010079 | DOI Listing |
Int J Microbiol
July 2025
Department of General Biology, Montes Claros State University, Montes Claros, Minas Gerais, Brazil.
The Brazilian savannah, Cerrado, a Brazilian phytophysiognomy, is an entire biome that contains as well as other Brazilian ecosystems, a vast biodiversity of microorganisms associated with native plants. Plant species of the Cerrado have attracted attention due to the still limited knowledge regarding their associated microbiota and the possible applications of these microorganisms. Considering that wildflowers are rich reservoirs of yeast diversity, the present study isolated 58 yeast strains from flowers of from two areas of Cerrado in northern Minas Gerais, Brazil.
View Article and Find Full Text PDFMolecules
May 2025
Department of Biotechnology and Food Analysis, Faculty of Production Engineering, Wroclaw University of Economics and Business, Komandorska 118/120, 53-345 Wrocław, Poland.
As a complex of enzymes α-l-rhamnosidase and β-d-glucosidase, naringinase catalyzes the deglycosylation of flavonoids. According to the PRISMA method, a systematic literature review was conducted analyzing peer-reviewed scientific articles from the Scopus and Web of Science databases. Recent reviews on naringinase have focused on its sources, production, and general applications, whereas the present study highlights its specific applications, its role in the deglycosylation of flavonoids, and the resulting improvement in their bioavailability.
View Article and Find Full Text PDFJ Fungi (Basel)
May 2025
Post Graduate Research Laboratory, Department of Botany, Smt. Chandibai Himathmal Mansukhani College of Arts, Science & Commerce, Ulhasnagar 421003, Thane, MS, India.
Tannase, a highly adaptive biocatalyst, plays a pivotal role in diverse bioconversion reactions in nature. This enzyme exhibits numerous applications across various industrial sectors, including food, pharmaceuticals, chemicals, and beverages. This study aimed to screen and characterize fungal endophytes isolated from mangrove plants for their enzyme tannase-producing ability.
View Article and Find Full Text PDFInt J Biol Macromol
June 2025
Scientific Department, St. Petersburg State Agrarian University, Pushkin 196601, Russia. Electronic address:
Tannases are a family of esterases that catalyze the hydrolysis of ester and depside bonds present in hydrolyzable tannins to release glucose and gallic acid. This enzyme is widely spread in animals, plants, and microbes. In particular, fungi and bacteria are the major sources of tannase.
View Article and Find Full Text PDFJ Agric Food Chem
February 2025
State Key Laboratory of Coordination Chemistry, Key Laboratory of Mesoscopic Chemistry (Ministry of Education), School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.
Facile pesticide nanocapsules were successfully prepared by directly encapsulating the antisolvent precipitation of pesticides through instantaneous "on site" coordination assembly of tannic acid and Fe, avoiding tedious preparation, time consumption, and large amounts of organic solvents. The pesticide nanocapsules showed excellent resistance to ultraviolet photolysis and rainwater washing owing to the nanocapsule walls. The smart pesticide nanocapsules exhibited the controlled release of pesticides under multidimensional stimuli, such as acidic/alkaline pH, glutathione, HO, phytic acid, laccase, tannase, and sunlight, which were related to the physiological and natural environments of crops, pests, and pathogens.
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