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[This corrects the article DOI: 10.1371/journal.pone.0311838.].
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Front Bioeng Biotechnol
August 2025
Institute of Process Engineering in Life Sciences, Section IV: Biomolecular Separation Engineering, Karlsruhe Institute of Technology (KIT), Karlsruhe, Germany.
Spectroscopic soft sensors are developed by combining spectral data with chemometric modeling, and offer as Process Analytical Technology (PAT) tools powerful insights into biopharmaceutical processing. In this study, soft sensors based on Raman spectroscopy and linear or partial least squares (PLS) regression were developed and successfully transferred to a filtration-based recovery step of precipitated virus-like particles (VLPs). For near real-time monitoring of product accumulation and precipitant depletion, the dual-stage cross-flow filtration (CFF) set-up was equipped with an on-line loop in the second membrane stage.
View Article and Find Full Text PDFNat Chem Biol
September 2025
Department of Biochemistry and Molecular Biology, School of Basic Medicine, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
J Clin Invest
August 2025
Department of Pediatrics, Washington University School of Medicine, St. Louis, United States of America.
Neurosurg Rev
August 2025
Beijing Neurosurgical Institute, Capital Medical University, Beijing, 100070, China.
Front Pharmacol
August 2025
Institute of Dermatology, Guangzhou Medical University, Guangzhou, China.
Background: Acne vulgaris is a prevalent chronic inflammatory disorderof the skin and oral isotretinoin is one of the most effective treatments forsevere acne with incompletely understood mechanisms. The aim of thisstudy was to investigate the pathogenesis of acne and the therapeuticmechanisms underlying isotretinoin treatment from integrated human plasma metabolomics and proteomics.
Methods: Liquid chromatography-tandem mass spectrometry (LC-MS/MS) full-spectrum metabolomics and four-dimensional data-independent acquisition (4D-DIA) quantitative proteomics were employed to analyze plasma samples from patients with group AG (severe acne group), group AG1 (severe acne group1, before isotretinoin treatment), group TG (isotretinoin treatment group) and group CG (control group).