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Background: No clear agreement exists on the degree of bone formation required to remove a metal plate without correction loss after medial opening-wedge high tibial osteotomy (MOWHTO). We aimed to investigate the mechanical stability of the proximal tibia with different bone formations after plate removal in MOWHTO using finite element models and determine the extent of bone formation when the plate can be removed without correction loss.
Methods: The MOWHTO models with 5, 10, and 15 mm opening gaps were generated. The mechanical stability of proximal tibial models with different extents of bone formation (from the lateral cortex of the osteotomy wedge to 20% (zone 1), 40% (zone 2), 50% (zone 2.5), 60% (zone 3), 70% (zone 3.5), 80% (zone 4), and 100% bone formation medially) after plate removal was analyzed using finite element analysis. Bone stress and strain and micromotion were evaluated to investigate fracture risk and bone stability, respectively, in various types of tibial models.
Results: Peak von Mises stress was lower than yield strength when bone formation reached zone 3.5 (70%) or more in 5- and 10-mm osteotomy gap models, and zone 4 (80%) or more in a 15-mm gap model. Maximal principal strains were lower than 6,130 microstrain when bone formation reaches zone 3.5 (70%) or more in models with osteotomy gaps of 5, 10, and 15 mm.
Conclusions: This indicates that plate removal without correction loss after MOWHTO may be possible when bone formation reaches zone 3.5 (> 70%) or more during 5- and 10-mm osteotomy gap corrections, and zone 4 (> 80%) or more during 15-mm gap correction. The present study results suggest that it would be safer to perform plate removal after obtaining sufficient bone formation rather than performing it near the osteotomy gap center (50%) to avoid correction loss considering both coronal and sagittal plane aspects.
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http://dx.doi.org/10.1186/s13018-024-05373-9 | DOI Listing |
J Am Soc Nephrol
September 2025
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN, USA.
Background: Genetic modifiers are believed to play an important role in the onset and severity of polycystic kidney disease (PKD), but identifying these modifiers has been challenging due to the lack of effective methodologies.
Methods: We generated zebrafish mutants of IFT140, a skeletal ciliopathy gene and newly identified autosomal dominant PKD (ADPKD) gene, to examine skeletal development and kidney cyst formation in larval and juvenile mutants. Additionally, we utilized ift140 crispants, generated through efficient microhomology-mediated end joining (MMEJ)-based genome editing, to compare phenotypes with mutants and conduct a pilot genetic modifier screen.
Eur Arch Paediatr Dent
September 2025
Araçatuba School of Dentistry, São Paulo State University - UNESP, Araçatuba, Brazil.
Purpose: This systematic review provides a critical evaluation, synthesis of the existing literature on isotretinoin's effects on craniomaxillofacial bone.
Methods: Following the PRISMA guidelines and registered in PROSPERO, the review was conducted in August 2024 across various databases. Eligible in vivo studies were analysed for their assessment of isotretinoin's effects on craniomaxillofacial bone.
Osteoporos Int
September 2025
Molecular Bone Histology Lab, Research Unit of Pathology, Department of Clinical Research, University of Southern Denmark, Odense, Denmark.
Intermittent PTH treatment has been used as both an osteoanabolic treatment in osteoporosis and a hormone replacement in hypoparathyroidism for many years. This scoping review compiles and reinterprets studies using histomorphometry supported by bone turnover markers to investigate the elusive cellular effect of intermittent PTH treatment locally within the bone, while illuminating knowledge gaps. Intermittent PTH increases both osteoclast and osteoblast activity within the first 6 months of treatment.
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September 2025
Stomatology Hospital, School of Stomatology, Zhejiang University School of Medicine, Zhejiang Provincial Clinical Research Center for Oral Diseases, Key Laboratory of Oral Biomedical Research of Zhejiang Province, Cancer Center of Zhejiang University, Engineering Research Center of Oral Biomaterials
The onset and progression of periodontitis are closely related to metabolic reprogramming in the periodontal microenvironment, with osteoclasts playing a critical role in tissue destruction. Single-cell RNA sequencing (scRNA-seq) of periodontal tissues from healthy individuals and patients with severe chronic periodontitis revealed a significant increase in the expression of mitochondrial-related genes during osteoclast differentiation, suggesting the critical role of mitochondrial function in this process. This study investigates the potential of the novel mitoribosome-targeting antibiotic radezolid in inhibiting osteoclast differentiation.
View Article and Find Full Text PDFACS Synth Biol
September 2025
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, P. R. China.
Human Bone Morphogenetic Protein-2 (hBMP-2) serves as a critical regulator in bone and cartilage formation; however, its industrial application is hindered by its inherent tendency to form inclusion bodies in prokaryotic expression systems. To address this issue, we established a recombinant hBMP-2 (rhBMP-2) expression system using the pCold II plasmid and the SHuffle T7 strain. We explored several strategies to enhance the solubility of rhBMP-2, including coexpression with molecular chaperones, vesicle-mediated secretory expression, fusion expression with synthetic intrinsically disordered proteins (SynIDPs), and fusion expression with small-molecule peptide tags.
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