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Article Abstract
Delivering microRNA (miRNA) to treat cardiac disease is a significant challenge, and selecting an efficient delivery method to target the affected organ is critical for therapeutic success. Here, we present a protocol for delivering miRNA to the heart of rats with heart failure using adeno-associated virus (AAV9) coupled with a hydrodynamic transfusion strategy. We describe steps for inducing heart failure, echocardiography, and AAV9 application. Further, we provide a comprehensive procedure to detect miRNA expression in cardiac tissues using stem-loop RT-qPCR. For complete details on the use and execution of this protocol, please refer to Mushtaq et al..
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699408 | PMC |
| http://dx.doi.org/10.1016/j.xpro.2024.103498 | DOI Listing |
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