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During the 2022 and ongoing mpox outbreak, PCR diagnostics were already within the Laboratory Response Network (LRN) laboratories and additionally rapidly deployed to detect monkeypox (mpox) virus in patients and aid in accurate diagnosis. This accuracy is dependent on proper collection, shipment, and adherence to PCR procedure. This book chapter discusses effective collection, storage, and transportation for a real-time PCR to detect monkeypox virus nucleic acid.
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http://dx.doi.org/10.1007/978-1-0716-4160-6_2 | DOI Listing |
PLoS One
September 2025
Satellite Collections North, Genebank Department, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Malchow/Poel, Germany.
Treatment of seeds with cold atmospheric pressure plasma (CAPP) is in its proof-of-concept phase with regard to its effect on germination and plant growth. To increase the germination of hardseeded red clover (Trifolium pratense L.), seeds are usually scarified, which is time-consuming and labour-intensive.
View Article and Find Full Text PDFPLoS One
September 2025
Department of Environmental and Occupational Health and Safety, Institute of Public Health, College of Medicine and Other Health Sciences, University of Gondar, Gondar, Ethiopia.
Foodborne diseases pose a significant public health challenge worldwide. The increasing availability of edible oils in the market, combined with Ethiopia's lack of stringent quality control and regulatory oversight, raises concerns about their safety. This inadequacy in regulation may contribute to microbial contamination, leading to potential public health risks.
View Article and Find Full Text PDFEur J Clin Pharmacol
September 2025
Department of Hospital Pharmacy, Meander Medical Centre, Amersfoort, the Netherlands.
Purpose: This study was designed to analyse the influence of temperature, pH and storage time on unbound fractions of PHT and VPA.
Methods: The influence of ultrafiltration (UF) temperature on measured unbound fractions of PHT and VPA in spiked samples was evaluated in a single laboratory experiment and in data from a national external quality control (EQC) database. The influence of pH adjustment with phosphate buffered saline (PBS) on measured unbound fractions of PHT and VPA was investigated in patient samples.
J Acoust Soc Am
September 2025
Applied Physics Laboratory, University of Washington, Seattle, Washington 98105, USA.
Echolocating bats provide vital ecosystem services and can be monitored effectively using passive acoustic monitoring (PAM) techniques. Duty-cycle subsampling is widely used to collect PAM data at regular ON/OFF cycles to circumvent battery and storage capacity constraints for long-term monitoring. However, the impact of duty-cycle subsampling and potential detector errors on estimating bat activity has not been systematically investigated for bats.
View Article and Find Full Text PDFActa Anaesthesiol Scand
October 2025
Copenhagen Trial Unit, Centre for Clinical Intervention Research, The Capital Region, Copenhagen University Hospital - Rigshospitalet, Copenhagen, Denmark.
Introduction: Electronic health records can be used to create high-quality databases if data are structured and well-registered, which is the case for most perioperative data in the Capital and Zealand Regions of Denmark. We present the purpose and development of the AI and Automation in Anaesthesia (TRIPLE-A) database-a platform designed for epidemiology, prediction, quality control, and automated research data collection.
Methods: Data collection from the electronic medical record (EPIC Systems Corporation, WI, USA) was approved by the Capital Region, Denmark, and ethical approval was waived.