A bifunctional MXene@PtPd NPs cascade DNAzyme-mediated fluorescence/colorimetric dual-mode biosensor for Pb determination.

Food Chem

College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou 450001, China.

Published: February 2025


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Article Abstract

Pb has numerous sources in cosmetics, industrial pollution and other environments. Therefore, sensitive and accurate detection of Pb content is extremely important in food safety. In this work, bifunctional nanomaterials TiC@PtPd NPs with fluorescence quenching effect and peroxidase activity were prepared by in situ growth of platinum‑palladium nanoparticles (PtPd NPs) on the surface of 2D material TiC. Combining the DNA enzyme recognition element with magnetic separation technology, we constructed a fluorescence/colorimetric dual-channel for the sensitive detection of Pb. Under the optimal conditions, the detection ranges of this fluorescence/colorimetric bimodal sensing strategy were 0.1-1000 nmol/L and 0.5-1000 nmol/L, respectively. The LOD of the fluorescence method was 23 pmol/L, and that of the colorimetric method was 74 pmol/L, and the results of the detection were visible to the naked eye. This dual-mode sensing method provides a new platform for accurate, reliable and visualized detection of Pb.

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http://dx.doi.org/10.1016/j.foodchem.2024.141845DOI Listing

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