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http://dx.doi.org/10.1016/j.str.2024.10.029 | DOI Listing |
Chem Biodivers
September 2025
Department of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk, Republic of Korea.
One of the most significant problems facing the scientific community in the 21st century is diabetes mellitus. There is an urgent need to create new powerful compounds that can fight this terrible disease because the number of instances of diabetes and drug-resistant diabetes is rising. We have synthesized a novel series of thiazole-derived thiadiazole-based Schiff base derivatives (1-10) in an effort to identify potential antidiabetic agents.
View Article and Find Full Text PDFSTAR Protoc
September 2025
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture, Northwest A&F University, Yangling 712100, China. Electronic address:
High-throughput chromosome conformation capture (Hi-C) provides genome-wide insights into chromatin interactions within the three-dimensional structure of the nucleus, making it a powerful tool for studying genome architecture. Here, we provide a modified in situ Hi-C protocol for small cell numbers, utilizing 50-100 embryonic cells at the 8-cell stage to investigate chromatin organization during bovine early embryonic development. This protocol overcomes the challenges of limited sample availability and offers valuable insights into chromatin dynamics during bovine early embryogenesis.
View Article and Find Full Text PDFJ Biomol NMR
September 2025
Department of Structural Biology, St. Jude Children's Research Hospital, Memphis, TN, 38105, USA.
Biomolecular dynamics in the microsecond-to-millisecond (µs-ms) timescale are linked to various biological functions, such as enzyme catalysis, allosteric regulation, and ligand recognition. In solution state NMR, Carr-Purcell-Meiboom-Gill (CPMG) relaxation dispersion experiments are commonly used to probe µs-ms timescale motions, providing detailed kinetic, thermodynamic, and mechanistic information at the atomic level. For investigating conformational dynamics in high-molecular-weight biomolecules, methyl groups serve as ideal probes due to their favorable relaxation properties, and C CPMG relaxation dispersion is widely employed for characterizing dynamics in selectively CH-labeled samples.
View Article and Find Full Text PDFACS Sens
September 2025
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.
Alpha-2-macroglobulin (A2M) is a critical biomarker implicated in inflammation, immune regulation, coagulation, and various pathological conditions such as liver fibrosis, neurodegenerative diseases, and cancers. However, its precise quantification remains challenging due to complex conformational dynamics, subtle abundance fluctuations, and interference from plasma proteins. Here, we present a label-free dynamic single-molecule sensing (LFDSMS) strategy for the sensitive and specific detection of A2M.
View Article and Find Full Text PDFNucleic Acids Res
September 2025
Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, Brno 61200, Czech Republic.
RNA G-quadruplexes (rG4s) are emerging as vital structural elements involved in processes like gene regulation, translation, and genome stability. Found in untranslated regions of messenger RNAs (mRNAs), they influence translation efficiency and mRNA localization. Additionally, rG4s of long noncoding RNAs and telomeric RNA play roles in RNA processing and cellular aging.
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