Article Synopsis
- Hi-C is a technique used to study the three-dimensional structure of chromatin, aiding in genome assembly.
- GutHi-C is a newly developed method that is efficient and easy to use for analyzing gut microorganisms in humans, livestock, and poultry.
- It serves as a reference point for enhancing the Hi-C methodology related to microbial metagenomes.
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Article Abstract
Hi-C can obtain three-dimensional chromatin structure information and is widely used for genome assembly. We constructed the GutHi-C technology. As shown in the graphical abstract, it is a highly efficient and quick-to-operate method and can be widely used for human, livestock, and poultry gut microorganisms. It provides a reference for the Hi-C methodology of the microbial metagenome. DPBS, Dulbecco's phosphate-buffered saline; Hi-C, high-through chromatin conformation capture; LB, Luria-Bertani; NGS, next-generation sequencing; PCR, polymerase chain reaction; QC, quality control.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11487548 | PMC |
| http://dx.doi.org/10.1002/imt2.227 | DOI Listing |
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