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Ingestible microdevices represent a breakthrough in non-invasive sampling of the human gastrointestinal (GI) tract. By capturing the native spatiotemporal microbiome and intricate biochemical gradients, these devices allow a non-invasive multi-omic access to the unperturbed host-microbiota crosstalk, immune/nutritional landscapes and gut-organ connections. We present the current progress of GI sampling microdevices towards personalized metabolism and fostering collaboration among clinicians, engineers, and data scientists.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC11447266 | PMC |
http://dx.doi.org/10.1038/s41522-024-00536-2 | DOI Listing |
Med Oncol
September 2025
Chemistry Department, Faculty of Science, Cairo University, Cairo, Egypt.
Pancreatic cancer is among the most lethal forms of cancer, with a five-year survival rate under 7%, primarily due to its late clinical presentation and rapid disease progression. Although the oncogenic development of pancreatic tumors can span over a decade, early diagnosis remains a major clinical challenge, as current diagnostic approaches-including imaging modalities and blood-based markers like CA19-9-lack the requisite sensitivity for detecting early-stage disease. Liquid biopsy has emerged as a promising, non-invasive diagnostic technique by enabling the detection of circulating tumor-specific nucleic acids, particularly circulating tumor DNA (ctDNA) and microRNAs (miRNAs).
View Article and Find Full Text PDFAnalyst
September 2025
Department of BioNano Technology, Gachon University, 1342 Seongnam-daero, Sujeong-gu, Seongnam-si 13120, Gyeonggi-do, Korea.
Herein, we introduce a LEGO®-inspired molecular diagnostic microdevice fully integrated with DNA extraction, loop-mediated isothermal amplification (LAMP), and colorimetric detection functionalities for rapid detection of antibacterial resistance in a pipette-free manner. The microdevice system is composed of a ready-to-use microdevice containing all necessary reagents and stamps that offer sample-to-answer diagnosis in a pipette-free manner. In particular, antimicrobial resistance was analyzed through LAMP at a significantly reduced temperature of approximately 40 °C, combined with DNA extraction and detection, which were performed at room temperature (RT).
View Article and Find Full Text PDFBiosens Bioelectron
December 2025
School of Instrument Science and Technology, State Key Laboratory for Manufacturing System Engineering, Xi'an Jiaotong University, Xi'an, 710049, Shaanxi, China; State Industry-Education Integration Center for Medical Innovations, Xi'an Jiaotong University, Xi'an, 710054, Shaanxi, China; Key Laborat
Clustered regularly interspaced short palindromic repeats (CRISPR)-based nucleic acid detection has advanced rapidly due to its simplicity, high specificity, and high sensitivity. However, most conventional CRISPR-based detection methods lack quantitative capability. Although the strategy of digital PCR technology enhances the sensitivity of CRISPR detection and enables quantification analysis through sample dispersion, integration remains limited.
View Article and Find Full Text PDFBiomed Microdevices
August 2025
Department of Biomedical Sciences, Humanitas University, Via Rita Levi Montalcini 4, Pieve Emanuele, 20072, Italy.
Periprosthetic joint infection (PJI) is a serious complication of prosthetic joint implantation, which poses a significant burden on both individuals and society. Effective treatment relies on the rapid identification of the underlying cause; however, the diagnosis of PJI remains challenging, inefficient, and time-consuming. Current detection protocols based on clinical signs and conventional cultures often fail to provide definitive results.
View Article and Find Full Text PDFOpen Forum Infect Dis
July 2025
Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.
Background: We recently described the utility of the "TB Concentration & Transport" kit for biosafe, ambient temperature transport of dried sputum on -Filter, and the "TB DNA Extraction" kit for DNA extraction from -Filter for the early diagnosis of drug-resistant tuberculosis (TB). This study aimed to assess the feasibility and compatibility of these kits with line probe assay (LPA) under National Tuberculosis Elimination Programme (NTEP) settings.
Methods: Patients with presumptive pulmonary TB, multidrug-resistant (MDR) TB, or extensively drug-resistant TB (N = 8491) who attended Designated Microscopy Centers (DMCs, n = 13) under National Reference Laboratories (NRLs) at Bhopal, New Delhi, Chennai, and Bhubaneswar were screened by smear microscopy.